PXD004289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Site-specific glycosylation of donkey milk lactoferrin investigated by High Resolution Mass Spectrometry |
Description | A comprehensive glycosylation profile of donkey lactoferrin, isolated by ion exchange chromatography from an individual milk sample, was obtained by means of chymotryptic digestion, TiO2 and HILIC enrichment, reversed-phase high performance liquid chromatography, electrospray mass spectrometry, and high collision dissociation fragmentation. The results obtained allowed the identification of 26 different glycan structures, including high mannose, complex and hybrid N-glycans, linked to the protein backbone via an amide bond to asparagine residues located at the positions 137, 281 and 476. Altogether, the N-glycan structures determined revealed that in donkey milk lactoferrin most of the N-glycans identified are neutral complex/hybrid. Actually, 10 neutral non-fucosylated complex/hybrid N-glycans and 4 neutral fucosylated complex/hybrid N-glycans were found. In addition, 2 high mannose N-glycans, 4 sialylated fucosylated complex/hybrid N-glycans and 6 sialylated non-fucosylatedN-glycans, one of which containing N-glycolylneuramin acid (Neu5Gc), were found. A comparison of the glycosylation profile of donkey milk lactoferrin with respect to that of human, bovine and goat milk lactoferrin is reported. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-25 |
AnnouncementXML | Submission_2016-08-25_00:41:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Salvatore Foti |
SpeciesList | scientific name: Equus asinus (Donkey); NCBI TaxID: 9793; |
ModificationList | complex glycosylation |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-08 04:04:32 | ID requested | |
⏵ 1 | 2016-08-25 00:41:22 | announced | |
Publication List
Gallina S, Saletti R, Cunsolo V, Muccilli V, Foti S, Roepstorff P, Rasmussen MI, Site-specific glycosylation of donkey milk lactoferrin investigated by high-resolution mass spectrometry. Amino Acids, 48(12):2799-2808(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Lactoferrin glycosylation · Donkey milk · N-glycan structures · TiO2 and HILIC enrichment · Mass spectrometry |
Contact List
Salvatore Foti |
contact affiliation | Organic Mass Spectrometry Laboratory, Department of Chemical Sciences, University of Catania, Viale A. Doria 6, I-95125 Catania, Italy |
contact email | sfoti@unict.it |
lab head | |
Salvatore Foti |
contact affiliation | University of Catania |
contact email | sfoti@unict.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004289
- Label: PRIDE project
- Name: Site-specific glycosylation of donkey milk lactoferrin investigated by High Resolution Mass Spectrometry