PXD004285

PXD004285 is an original dataset announced via ProteomeXchange.

Title	Non-intein splicing of RB47
Description	The poly A binding protein RB47 was purified from C. reinhardtii chloroplasts and was found to have endoribonuclease activity. MS analysis suggested that an internal non-conserved linker (NCL)sequence was absent from the native protein. Recombinant, full length RB47 protein, including the NCL did not have endoribonuclease activity while versions lacking the NCL were active. An in vitro system, utilizing recombinant RB47 and chloroplast lysates, recreated the processing of RB47 in that the NCL was excised and the flanking sequences were spliced together.
HostingRepository	PRIDE
AnnounceDate	2018-10-27
AnnouncementXML	Submission_2018-10-26_16:10:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Campbell
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-07 01:19:11	ID requested
⏵ 1	2018-10-26 16:10:09	announced

Campbell SJ, Stern DB, Activation of an Endoribonuclease by Non-intein Protein Splicing. J Biol Chem, 291(31):15911-15922(2016) [pubmed]

curator keyword: Biological

submitter keyword: Intein, RB47, C. reinhardti, chloroplast

David Stern
contact affiliation	Boyce Thompson Institute
contact email	ds28@cornell.edu
lab head
Stephen Campbell
contact affiliation	Boyce Thompson Institute
contact email	sjc53@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004285

PRIDE project URI

• PRIDE
  1. PXD004285
     1. Label: PRIDE project
     2. Name: Non-intein splicing of RB47