PXD004283

PXD004283 is an original dataset announced via ProteomeXchange.

Title	Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
Description	Sepsis caused by Gram-positive bacterial pathogens is a major fatal disease but its molecular basis remains elusive. Toll-like receptor 2 (TLR2) has been implicated in the orchestration of inflammation and sepsis but its role appears to vary for different pathogen species and clones. Accordingly, Staphylococcus aureus clinical isolates differ substantially in their capacity to activate TLR2. Here we show that strong TLR2 stimulation depends on high-level production of phenol-soluble modulin (PSM) peptides in response to the global virulence activator Agr. PSMs are required for mobilizing lipoproteins, the TLR2 agonists, from the staphylococcal cytoplasmic membrane. Notably, the course of sepsis caused by PSM-deficient S. aureus is similar in wild-type and TLR2-deficient mice, but TLR2 is required for protection of mice against PSM-producing S. aureus. Thus, a crucial role of TLR2 depends on agonist release by bacterial surfactants. Modulation of this process may lead to new therapeutic strategies against Gram-positive infections.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:59.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-06 07:27:12	ID requested
1	2016-06-08 05:30:34	announced
2	2016-08-02 05:57:55	announced	Updated publication reference for PubMed record(s): 27470911.
⏵ 3	2024-10-22 04:30:04	announced	2024-10-22: Updated project metadata.

10.1038/ncomms12304;

Hanzelmann D, Joo HS, Franz-Wachtel M, Hertlein T, Stevanovic S, Macek B, Wolz C, G, ö, tz F, Otto M, Kretschmer D, Peschel A, Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants. Nat Commun, 7():12304(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Phenol-soluble modulins, lipopeptide, culture supernatant, quantitative label-free proteomics, exoproteome,Staphylococcus aureus

Andreas Peschel
contact affiliation	Interfaculty Institute of Microbiology and Infection Medicine, Infection Biology Department, University of Tübingen, Auf der Morgenstelle 28 72076 Tübingen, Germany
contact email	andreas.peschel@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	University of Rouen
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004283

PRIDE project URI

• PRIDE
  1. PXD004283
     1. Label: PRIDE project
     2. Name: Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants