PXD004280

PXD004280 is an original dataset announced via ProteomeXchange.

Title	Quantitative Secretome Analysis of Activated Jurkat Cells
Description	A quantitative label-free secretome analysis protocol using a click chemistry-based approach for the enrichment of secreted glycoproteins was adapted for and applied to a T cell model. There, Jurkat cells were activated via PMA/ionomycin and the dynamic modulation of the T cell secretome was investigated and compared to the dynamic modulation of the T cell proteome of the same model.
HostingRepository	PRIDE
AnnounceDate	2016-10-10
AnnouncementXML	Submission_2016-10-10_09:24:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004280
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thilo Bracht
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-histidine residue; Propionamide; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-06 03:06:05	ID requested
⏵ 1	2016-10-10 09:24:09	announced

Witzke KE, Rosowski K, M, ü, ller C, Ahrens M, Eisenacher M, Megger DA, Knobloch J, Koch A, Bracht T, Sitek B, Quantitative Secretome Analysis of Activated Jurkat Cells Using Click Chemistry-Based Enrichment of Secreted Glycoproteins. J Proteome Res, 16(1):137-146(2017) [pubmed]

curator keyword: Biological

submitter keyword: Secretome, Jurkat, T cell model, quantitative label-free LC-MS/MS

Barbara Sitek
contact affiliation	Clinical Proteomics Medizinisches Proteom-Center Ruhr-Universität Bochum Universitätsstraße 150 44801 Bochum
contact email	barbara.sitek@rub.de
lab head
Thilo Bracht
contact affiliation	Clinical Proteomics - Cancer Research
contact email	thilo.bracht@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD004280

PRIDE project URI

• PRIDE
  1. PXD004280
     1. Label: PRIDE project
     2. Name: Quantitative Secretome Analysis of Activated Jurkat Cells