PXD004276

PXD004276 is an original dataset announced via ProteomeXchange.

Title	Circadian profiling of the Arabidopsis proteome using 2D-DIGE
Description	Clock-generated biological rhythms provide an adaptive advantage to an organism which results in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under constant light. Using large-scale two-dimensional differential in-gel electrophoresis (2D-DIGE) the abundance of more than one thousand proteins spots was reproducibly resolved, quantified and profiled across a circadian time series. A comparison between phenol-extracted samples and Rubisco-depleted extracts identified 70 and 39 rhythmically-expressed proteins, respectively.
HostingRepository	PRIDE
AnnounceDate	2016-08-03
AnnouncementXML	Submission_2016-08-03_03:58:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hirofumi Nakagami
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-06 01:24:54	ID requested
1	2016-06-29 06:46:11	announced
⏵ 2	2016-08-03 03:58:23	announced	Updated publication reference for PubMed record(s): 27462335.

Choudhary MK, Nomura Y, Shi H, Nakagami H, Somers DE, Circadian Profiling of the Arabidopsis Proteome Using 2D-DIGE. Front Plant Sci, 7():1007(2016) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, circadian clock, 2D-DIGE

Hirofumi Nakagami
contact affiliation	Plant Proteomics Research Unit, RIKEN CSRS
contact email	hirofumi.nakagami@riken.jp
lab head
Hirofumi Nakagami
contact affiliation	RIKEN
contact email	hirofumi.nakagami@riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004276

PRIDE project URI

• PRIDE
  1. PXD004276
     1. Label: PRIDE project
     2. Name: Circadian profiling of the Arabidopsis proteome using 2D-DIGE