PXD004266

PXD004266 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HEK cell MYO7A-PDZD7 immunoprecipitation
Description	Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate low-abundance MYO7A protein complexes from those membranes, including one with the scaffolding protein PDZD7. To confirm the interaction of PDZD7 and MYO7A, we expressed them singly or together in HEK cells, and immunoprecipitated detergent-soluble fractions with specific antibodies. PDZD7 and MYO7A interacted strongly in these tissue-culture cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:49.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barr-Gillespie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-02 01:39:42	ID requested
1	2016-08-16 06:39:53	announced
⏵ 2	2024-10-22 04:29:50	announced	2024-10-22: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

curator keyword: Biological
submitter keyword: myosin,Tissue culture cells, PDZ, immunoprecipitation

curator keyword: Biological

submitter keyword: myosin,Tissue culture cells, PDZ, immunoprecipitation

Contact List

Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239
contact email	gillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004266
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004266

PRIDE project URI

Repository Record List

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