PXD004254 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of potential human PML interacting proteins in Nicotiana benthamiana |
Description | In spite of the crucial tasks fulfilled by the subnuclear structures known as PML nuclear bodies (PML-NBs) in mammalian cells, the presence of PML-NBs in plant cells could not be shown yet. Interestingly, the key organizer of PML-NBs, human promyelocytic leukemia protein (PML), is not targeted to structures resembling PML-NBs when expressed heterologously in plants, but localizes to interchromosomal areas associated with the nucleolus. In order to identify plant proteins interacting with human PML, a GFP-fusion protein was ectopically expressed in Nicotiana benthamiana using Agrobacterium tumefaciens mediated transformation. The bait protein as well as potential interactors were immunoprecipitated by use of magnetic GFP-Trap beads (Chromotek). Precipitated proteins were analyzed by Nano-LC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:30:03.252.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Lamm |
SpeciesList | scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-31 03:07:05 | ID requested | |
1 | 2016-10-10 00:46:25 | announced | |
⏵ 2 | 2024-10-22 04:30:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Nicotiana benthamiana, PML nuclear bodies, coimmunoprecipitation, Nano-LC MS/MS, human promyelocytic leukemia protein |
Contact List
Uwe Sonnewald |
contact affiliation | Division of Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstraße 5, 91058 Erlangen |
contact email | Uwe.Sonnewald@FAU.de |
lab head | |
Christian Lamm |
contact affiliation | Friedrich-Alexander Universität Erlangen-Nürnberg |
contact email | Christian.lamm@fau.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004254
- Label: PRIDE project
- Name: Identification of potential human PML interacting proteins in Nicotiana benthamiana