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PXD004254

PXD004254 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of potential human PML interacting proteins in Nicotiana benthamiana
DescriptionIn spite of the crucial tasks fulfilled by the subnuclear structures known as PML nuclear bodies (PML-NBs) in mammalian cells, the presence of PML-NBs in plant cells could not be shown yet. Interestingly, the key organizer of PML-NBs, human promyelocytic leukemia protein (PML), is not targeted to structures resembling PML-NBs when expressed heterologously in plants, but localizes to interchromosomal areas associated with the nucleolus. In order to identify plant proteins interacting with human PML, a GFP-fusion protein was ectopically expressed in Nicotiana benthamiana using Agrobacterium tumefaciens mediated transformation. The bait protein as well as potential interactors were immunoprecipitated by use of magnetic GFP-Trap beads (Chromotek). Precipitated proteins were analyzed by Nano-LC-MS/MS.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:30:03.252.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Lamm
SpeciesList scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-31 03:07:05ID requested
12016-10-10 00:46:25announced
22024-10-22 04:30:03announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Nicotiana benthamiana, PML nuclear bodies, coimmunoprecipitation, Nano-LC MS/MS, human promyelocytic leukemia protein
Contact List
Uwe Sonnewald
contact affiliationDivision of Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstraße 5, 91058 Erlangen
contact emailUwe.Sonnewald@FAU.de
lab head
Christian Lamm
contact affiliationFriedrich-Alexander Universität Erlangen-Nürnberg
contact emailChristian.lamm@fau.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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