PXD004253

PXD004253 is an original dataset announced via ProteomeXchange.

Title	Nano-liquid chromatography-orbitrap MS/MS-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells
Description	A shotgun proteomics study based on stable isotope dimethyl labeling (DML) and nano liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) has been performed to identify the relative changes in proteins and to gain some light on the specific molecular targets and mechanisms of action of CA and CS in HT-29 colon cancer cells.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_06:12:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Valdés
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	4x(2)H labeled dimethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-31 02:58:18	ID requested
1	2016-12-23 13:38:36	announced
2	2017-06-20 05:44:03	announced	Updated project metadata.
3	2017-06-21 00:31:26	announced	Updated project metadata.
⏵ 4	2017-10-24 06:12:40	announced	Updated project metadata.

Vald, é, s A, Garc, í, a-Ca, ñ, as V, Artemenko KA, Sim, ó C, Bergquist J, Cifuentes A, Nano-liquid Chromatography-orbitrap MS-based Quantitative Proteomics Reveals Differences Between the Mechanisms of Action of Carnosic Acid and Carnosol in Colon Cancer Cells. Mol Cell Proteomics, 16(1):8-22(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: antiproliferative activity, carnosic acid, carnosol, colon cancer, dimethyl labeling, 16 foodomics, HT-29 cells, mass spectrometry, quantitative proteomics

Alejandro Cifuentes
contact affiliation	Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Calle Nicolás Cabrera 9, 28049, Madrid, Spain
contact email	a.cifuentes@csic.es
lab head
Alberto Valdés
contact affiliation	Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Calle Nicolás Cabrera 9, 28049, Madrid, Spain
contact email	a.valdes@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004253

PRIDE project URI

• PRIDE
  1. PXD004253
     1. Label: PRIDE project
     2. Name: Nano-liquid chromatography-orbitrap MS/MS-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells