PXD004252

PXD004252 is an original dataset announced via ProteomeXchange.

Title	Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy
Description	Although many affinity adsorbents have been developed for phosphopeptides enrichment, high-specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ions coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono- and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. We demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. Finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from HepG2 cells.
HostingRepository	PRIDE
AnnounceDate	2016-08-03
AnnouncementXML	Submission_2016-08-03_04:52:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zheyi Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-31 02:34:37	ID requested
⏵ 1	2016-08-03 04:53:01	announced

Liu Z, Wang F, Chen J, Zhou Y, Zou H, Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy. J Chromatogr A, 1461():35-41(2016) [pubmed]

curator keyword: Technical

submitter keyword: multi-phosphopeptide enrichment, competitive substitution, pre-coordination, mass spectrometry, phosphoproteomics

Fangjun Wang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
contact email	wangfj@dicp.ac.cn
lab head
Zheyi Liu
contact affiliation	Doctor
contact email	zy_liu@dicp.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004252
     1. Label: PRIDE project
     2. Name: Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a novel competitive substitution strategy