PXD004242

PXD004242 is an original dataset announced via ProteomeXchange.

Title	Plasma Proteome Profiling Reveals the Effects of Weight Loss on the Apolipoprotein Family and Systemic Inflammation Status
Description	Sustained weight loss is the preferred intervention in a wide range of metabolic conditions, but the effects on an individual´s health state remain ill-defined and controversial. Here, we investigate the plasma proteomes of a cohort of 43 obese individuals that had undergone eight weeks of weight loss (average of 12%) followed by a year of weight maintenance. Using plasma proteome profiling, a mass spectrometry-based approach, we measured 1294 plasma samples to examine dynamic changes of hundreds of human plasma proteins, including the entire apolipoprotein family. Longitudinal monitoring of the cohort revealed individual-specific protein levels and functional connected proteins with correlated regulation over time. The wide-ranging effect of losing weight on the plasma proteome was reflected in 93 significantly affected proteins. The adipocyte secreted SERPINF1 and the apolipoprotein APOF1 were most significantly down and up regulated (p<10**13), although their fold-change was modest (-16% and +37%), respectively. Overall, markers of inflammation went down in nearly all study participants (39 of 42), however, there were diverse patterns of inflammation marker responses, allowing individual-specific interpretation of the effects of weight loss. Plasma proteome profiling can be used broadly to evaluate and monitor intervention in metabolic diseases.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:03:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-31 00:17:39	ID requested
1	2017-01-02 12:52:05	announced
2	2017-01-03 00:02:28	announced	Updated publication reference for PubMed record(s): 28007936.
⏵ 3	2017-10-24 04:03:13	announced	Updated project metadata.

Geyer PE, Wewer Albrechtsen NJ, Tyanova S, Grassl N, Iepsen EW, Lundgren J, Madsbad S, Holst JJ, Torekov SS, Mann M, Proteomics reveals the effects of sustained weight loss on the human plasma proteome. Mol Syst Biol, 12(12):901(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Plasma proteome profile, mass spectrometry, clinic, human, apolipoproteins, inflammation, C-reactive protein, weight loss, obesity, metabolic syndrome, blood analysis

Matthias Mann
contact affiliation	Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004242
     1. Label: PRIDE project
     2. Name: Plasma Proteome Profiling Reveals the Effects of Weight Loss on the Apolipoprotein Family and Systemic Inflammation Status