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PXD004239

PXD004239 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAgave Americana Diel Proteome
DescriptionAgave americana 3 year-old clones were allowed to grow for one month under greenhouse 12h/12h light/dark conditions with regular irrigation. Three biological replicates of mature leaf were flash-frozen and ground under liquid nitrogen. Each cell pellet was resuspended in SDS buffer and sonicated. Proteins were extracted via TCA precipitation. Samples were denatured and reduced followed by enzymatic digestion using trypsin. Samples were analyzed via 2D (SCX-RP) nano-LC-MS/MS on an Orbitrap-Velos-Pro. MS/MS spectra were searched and filtered with MyriMatch and IDPicker. Note, the Peak List and Result files do not have the same sample description as found in the published manuscript. Please use the key provided in the Supplemental Files.
HostingRepositoryMassIVE
AnnounceDate2016-11-01
AnnouncementXMLSubmission_2016-11-01_08:42:27.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPaul Abraham
SpeciesList scientific name: Agave americana; NCBI TaxID: 39510;
ModificationListunknown modification: Carbamidomethyl Cysteine as static modification; unknown modification: Oxidation Methione and Carbamylation N-terminus as dynamic modification
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-27 07:13:32ID requested
12016-11-01 08:42:27announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CAM, proteomics, Agave, diel cycle
Contact List
Xiaohan Yang
lab head
Paul Abraham
contact affiliationOak Ridge Ridge National Laboratory
contact emailabrahampe@ornl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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