PXD004236

PXD004236 is an original dataset announced via ProteomeXchange.

Title	In vivo protein interaction network analysis reveals porin-localized toxin inactivation in antibiotic resistant bacteria
Description	The nosocomial pathogen Acinetobacter baumannii is a frequent cause of hospital acquired infections worldwide, and a challenge for treatment due to its evolved resistance to antibiotics, including carbapenems. To gain insight on A. baumannii antibiotic resistance mechanisms, we analyzed the protein interaction network of a multidrug-resistant A. baumannii clinical strain Ab5075. Using in vivo chemical cross-linking and mass spectrometry, we identified 2,068 non-redundant cross-linked peptide pairs containing 245 intra- and 398 inter- molecular interactions. Outer membrane proteins OmpA and YiaD, and carbapenemase Oxa-23 are hubs of the identified interaction network. Eighteen novel interactors of Oxa-23 were identified. Interactions of Oxa-23 with outer membrane porins OmpA and CarO were verified with co-immunoprecipitation analysis. Furthermore, transposon mutagenesis of oxa-23 or interactors of Oxa-23 demonstrated changes in meropenem or imipenem sensitivity in Ab5075. These results provide the first view of a porin-localized toxin inactivation model and increase understanding of bacterial antibiotic resistance mechanisms.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:17:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xia Wu
SpeciesList	scientific name: Acinetobacter baumannii AB5075; NCBI TaxID: 1116234;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-27 01:56:42	ID requested
1	2016-12-22 07:31:34	announced
⏵ 2	2017-10-24 03:17:23	announced	Updated project metadata.

Wu X, Chavez JD, Schweppe DK, Zheng C, Weisbrod CR, Eng JK, Murali A, Lee SA, Ramage E, Gallagher LA, Kulasekara HD, Edrozo ME, Kamischke CN, Brittnacher MJ, Miller SI, Singh PK, Manoil C, Bruce JE, In vivo protein interaction network analysis reveals porin-localized antibiotic inactivation in Acinetobacter baumannii strain AB5075. Nat Commun, 7():13414(2016) [pubmed]

curator keyword: Biological

submitter keyword: Acinetobacter baumannii, antibiotic resistance, carbapenem, chemical cross-linking, protein-protein interactions

James E. Bruce
contact affiliation	Department of Genome Sciences, University of Washington, Seattle, USA
contact email	jimbruce@uw.edu
lab head
Xia Wu
contact affiliation	University of Washington
contact email	xiawu2@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004236
     1. Label: PRIDE project
     2. Name: In vivo protein interaction network analysis reveals porin-localized toxin inactivation in antibiotic resistant bacteria