PXD004222 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | D10 Stereocilia Membrane Enrichment |
Description | Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate low-abundance MYO7A protein complexes from those membranes. The D10 stereocilia membrane enrichment protocol involves density centrifugation steps and immuno-enrichment of stereocilia using the D10 antibody, directed against the major stereocilia transmembrane protein PTPRQ. The data in this submission document the effectiveness of the enrichment procedure. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-16 |
AnnouncementXML | Submission_2016-08-16_06:26:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Barr-Gillespie |
SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-25 05:30:51 | ID requested | |
⏵ 1 | 2016-08-16 06:26:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Chicken, hair cells, stereocilia, hair bundles, inner ear, vestibular, auditory |
Contact List
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 |
contact email | gillespp@ohsu.edu |
lab head | |
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center |
contact email | gillespp@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004222 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004222
- Label: PRIDE project
- Name: D10 Stereocilia Membrane Enrichment