PXD004221 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Myosin VIIA 138-1 immunoaffinity purification |
Description | Through its activity in stereocilia, MYO7A (myosin VIIA) is essential for hair cell function in the inner ear. Utilizing multiple stages of immunoaffinity enrichment, we have developed a strategy that allows us to partially purify stereocilia membranes from thousands of chick inner ears and isolate low-abundance MYO7A protein complexes from those membranes. By analysis of MYO7A and co-purifying molecules with shotgun and targeted mass spectrometry, we demonstrated that MYO7A forms a complex with PDZD7, a paralog of USH1C and DFNB31, which has been shown to localize to the ankle-link region of stereocilia. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-16 |
AnnouncementXML | Submission_2016-08-16_06:09:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Barr-Gillespie |
SpeciesList | scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-25 05:30:26 | ID requested | |
⏵ 1 | 2016-08-16 06:09:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Hair cell, stereocilia, hair bundle, myosin, vestibular, auditory, PDZ |
Contact List
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 |
contact email | gillespp@ohsu.edu |
lab head | |
Peter Barr-Gillespie |
contact affiliation | Oregon Hearing Research Center |
contact email | gillespp@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004221 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004221
- Label: PRIDE project
- Name: Myosin VIIA 138-1 immunoaffinity purification