PXD004212

PXD004212 is an original dataset announced via ProteomeXchange.

Title	AP-MS analysis of sirtuin-2 interactions and whole-cell proteome analysis in human fibroblasts
Description	Sirtuins are NAD+-dependent deacylases with diverse enzymatic activities and substrates. They were implicated in regulation of cancer progression, neurodegeneration, aging, and viral infection. Sirtuin-2 localizes to the cytoplasm and is known to deacetylate alpha-tubulin. Current knowledge on sirtuin-2 interactions and its effect on gene expression in normal cells is very limited. We used proteomics approach to define its interactions and its effect on global protein levels in human fibroblasts.
HostingRepository	PRIDE
AnnounceDate	2016-08-12
AnnouncementXML	Submission_2016-08-12_02:05:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hanna Budayeva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-24 01:21:03	ID requested
⏵ 1	2016-08-12 02:05:09	announced

Budayeva HG, Cristea IM, Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking. Mol Cell Proteomics, 15(10):3107-3125(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: human fibroblasts, LC-MS/MS

Ileana M. Cristea
contact affiliation	Princeton University, Department of Molecular Biology
contact email	icristea@princeton.edu
lab head
Hanna Budayeva
contact affiliation	Princeton University
contact email	budayeva@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004212

PRIDE project URI

• PRIDE
  1. PXD004212
     1. Label: PRIDE project
     2. Name: AP-MS analysis of sirtuin-2 interactions and whole-cell proteome analysis in human fibroblasts