PXD004208

PXD004208 is an original dataset announced via ProteomeXchange.

Title	Different mechanisms of biotransformation of bisphenol A by the biphenyl-degrading Cupriavidus basilensis SBUG 290 lead to detoxification and reduction of estrogenicity
Description	A bacterial strain identified as Cupriavidus basilensis uses aromatic compounds as carbon and energy sources and has a high capability to transform the structurally related and hormonally active substance bisphenol A (BPA). Biphenyl-grown and phenol-grown cells converted BPA to five products within 24 h of incubation representing four different transformation pathways: (a) ring hydroxylation, (b) ring fission, (c) transamination and acetylation, and (d) dimerization. Products of the ring fission pathway were non-toxic and all five products exhibited a significantly reduced estrogenic activity compared to BPA. Cell cultivation in nutrient broth resulted in lower product quantities and dimerization was not proved. Thus the question arose whether enzymes of the biphenyl or phenol degradation pathway are involved in the transformation of BPA. Proteomic analyses revealed the constitutive expression of biphenyl degrading enzymes and indicated that the 2,3 dihydroxybiphenyl-1,2-dioxygenase might catalyse the meta-cleavage of the aromatic ring of BPA while enzymes of other pathways seemed to be involved in ring hydroxylation.
HostingRepository	PRIDE
AnnounceDate	2018-07-19
AnnouncementXML	Submission_2018-07-19_05:33:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Cupriavidus basilensis; NCBI TaxID: 68895;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-23 07:14:29	ID requested
⏵ 1	2018-07-19 05:33:44	announced

Z, ü, hlke MK, Schl, ü, ter R, Mikolasch A, Z, ü, hlke D, Giersberg M, Schindler H, Henning AK, Frenzel H, Hammer E, Lalk M, Bornscheuer UT, Riedel K, Kunze G, Schauer F, Biotransformation and reduction of estrogenicity of bisphenol A by the biphenyl-degrading Cupriavidus basilensis. Appl Microbiol Biotechnol, 101(9):3743-3758(2017) [pubmed]

curator keyword: Biological

submitter keyword: bisphenol A-degradation, LC-MS/MS

Katharina Riedel
contact affiliation	Institute for Microbiology University of Greifswald Friedrich-Ludwig-Jahnstr. 15 17489 Greifswald Germany
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004208
     1. Label: PRIDE project
     2. Name: Different mechanisms of biotransformation of bisphenol A by the biphenyl-degrading Cupriavidus basilensis SBUG 290 lead to detoxification and reduction of estrogenicity