PXD004195 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and phosphoproteomic profiling of ErbB2-dependent signalling |
Description | The mechanisms of ErbB2 signalling and the effects of its overexpression are not fully understood. Herein, SILAC expression profiling and phosphopeptide enrichment of a relevant, non-transformed, immortalized human mammary luminal epithelial cell model were used to profile ErbB2-dependent differences in protein expression and phosphorylation events triggered via EGFR (EGF treatment) and ErbB3 (HRGbeta1 treatment) in the context of ErbB2 overexpression. Bioinformatics analysis was used to infer changes in cellular processes and signalling events. We demonstrate the complexity of the responses to oncogene expression and growth factor signalling and identify protein changes relevant to ErbB2-dependent altered cellular phenotype, in particular cell cycle progression and hyper-proliferation, reduced adhesion and enhanced motility. Numerous novel sites of growth factor-regulated phosphorylation were also identified that were enhanced by ErbB2 overexpression and we putatively link these to altered cell behaviour. Moreover, we have defined a novel mechanism by which ErbB signalling suppresses basal interferon signalling that would promote the survival and proliferation of mammary luminal epithelial cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:29:52.693.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John Timms |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; phosphorylated residue; 6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-23 01:47:33 | ID requested | |
1 | 2018-10-26 07:16:38 | announced | |
⏵ 2 | 2024-10-22 04:29:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
Worthington J, Spain G, Timms JF, Effects of ErbB2 Overexpression on the Proteome and ErbB Ligand-specific Phosphosignaling in Mammary Luminal Epithelial Cells. Mol Cell Proteomics, 16(4):608-621(2017) [pubmed] |
10.1074/mcp.M116.061267; |
Keyword List
curator keyword: Biological |
submitter keyword: ErbB2 |
breast cancer |
LC-MS/MS |
SILAC |
phosphoproteomics |
Contact List
John Francis Timms |
contact affiliation | University College London |
contact email | john.timms@ucl.ac.uk |
lab head | |
John Timms |
contact affiliation | University College London |
contact email | john.timms@ucl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004195
- Label: PRIDE project
- Name: Proteomic and phosphoproteomic profiling of ErbB2-dependent signalling