PXD004190 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative GTPase affinity purification identifies Rho family protein interaction partners |
Description | Although Rho GTPases are essential molecular switches involved in many cellular processes, an unbiased experimental comparison of their interaction partners was not yet performed. Here, we develop quantitative GTPase affinity purification (qGAP) to systematically identify interaction partners of six Rho GTPases (Cdc42, Rac1, RhoA, RhoB, RhoC, RhoD) depending on their nucleotide loading state. We use Stable isotope labeling by Amino acids in cell culture (SILAC) and label free quantification (LFQ). Our interaction network contains many new proteins, reveals highly promiscuous binding of several effectors and mirrors evolutionary relationships of Rho GTPases. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:30:10.375.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Paul |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-19 05:43:07 | ID requested | |
1 | 2016-12-23 15:06:20 | announced | |
2 | 2017-10-24 06:12:18 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:30:15 | announced | 2024-10-22: Updated project metadata. |
Publication List
Paul F, Zauber H, von Berg L, Rocks O, Daumke O, Selbach M, Quantitative GTPase Affinity Purification Identifies Rho Family Protein Interaction Partners. Mol Cell Proteomics, 16(1):73-85(2017) [pubmed] |
Keyword List
submitter keyword: interaction proteomics,Rho GTPase |
Contact List
Dr Matthias Selbach |
contact affiliation | Max-Delbrück-Centrum für Molekulare Medizin (MDC) Robert-Rössle-Str. 10 13092 Berlin |
contact email | matthias.selbach@mdc-berlin.de |
lab head | |
Florian Paul |
contact affiliation | Max Delbrück Center |
contact email | florianpaul@gmx.net |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004190
- Label: PRIDE project
- Name: Quantitative GTPase affinity purification identifies Rho family protein interaction partners