PXD004187

PXD004187 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomic analysis of HIV-Infection Reveals Remodelling of the Cellular Phosphoproteome by Phylogenetically Diverse Lentiviral Vif Variants
Description	HIV accessory proteins manipulate host factors to evade cellular restriction and enhance viral replication. We used multiplex tandem mass tag (TMT)-based whole cell proteomics to gain a comprehensive, time-based overview of proteins and processes subverted during HIV infection. To make specific functional and mechanistic predictions and systematically identify candidate accessory protein targets, we categorized cellular proteins regulated by HIV according to their patterns of temporal expression. As well as depleting APOBEC family members, we found Vif to be necessary and sufficient for CUL5-dependent degradation of all members of the B56 family of regulatory subunits of the key cellular phosphatase PP2A. Quantitative phosphoproteomic analysis of HIV-infected cells confirmed Vif-dependent hyperphosphorylation of >200 cellular proteins, particularly substrates of the aurora kinases. The ability of Vif to target PP2A subunits spans primate and non-primate lentiviral lineages, and remodeling of the cellular phosphoproteome is therefore a second ancient and conserved Vif function.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:25.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yagnesh Umrania
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-19 01:39:45	ID requested
1	2016-09-29 07:38:50	announced
2	2016-10-05 03:50:56	announced	Updated publication reference for PubMed record(s): 27690223.
⏵ 3	2024-10-22 04:29:27	announced	2024-10-22: Updated project metadata.

10.7554/eLife.18296;

Greenwood EJ, Matheson NJ, Wals K, van den Boomen DJ, Antrobus R, Williamson JC, Lehner PJ, Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife, 5():(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: HIV, whole cell proteome, TMT, temporal

Paul J Lehner
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UK.
contact email	pjl30@cam.ac.uk
lab head
Yagnesh Umrania
contact affiliation	University of Cambridge
contact email	yu206@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004187

PRIDE project URI

• PRIDE
  1. PXD004187
     1. Label: PRIDE project
     2. Name: Temporal proteomic analysis of HIV-Infection Reveals Remodelling of the Cellular Phosphoproteome by Phylogenetically Diverse Lentiviral Vif Variants