PXD004186

PXD004186 is an original dataset announced via ProteomeXchange.

Title	Qualitative and quantitative analysis of Bothrops jararaca venom gland proteome
Description	While cellular transcripts encode rich information that provide key features to understand the molecular basis of snake venom variation, their presence/ abundance does not necessary imply/correlate in the translation of a functional (protein) product. In this study we carried out an analysis of the venom gland proteome of Bothrops jararaca taking into account two distinct phases of its ontogenetic development (i.e. newborn and adult specimens) and the marked sexual dimorphism recently reported on its venom proteome. Proteomic data analysis showed wider dynamic range for toxins when comparing to non-toxins and a dynamic proteome rearrangement in cellular proteins upon B. jararaca development. Differentially expressed proteins covered a number of biological pathways related to protein synthesis, including proteins related to transcription and translation, which were found to be significantly higher expressed in the newborn venom gland. Our results showed that the variation in the expression levels of cellular proteins gives rise to an even higher variation in the dynamic range of the expressed toxins. Upon ageing, the molecular constraints related to protein synthesis together with ecological traits would likely have an impact on the toxin repertoire, which, in the case of B. jararaca species, would enable the species to deal with different prey types during its lifespan.
HostingRepository	PRIDE
AnnounceDate	2016-09-28
AnnouncementXML	Submission_2016-09-28_05:05:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Zelanis
SpeciesList	scientific name: Bothrops jararaca (Jararaca) (Bothrops jajaraca); NCBI TaxID: 8724;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-19 01:36:05	ID requested
⏵ 1	2016-09-28 05:05:26	announced

Augusto-de-Oliveira C, Stuginski DR, Kitano ES, Andrade-Silva D, Liberato T, Fukushima I, Serrano SM, Zelanis A, Dynamic Rearrangement in Snake Venom Gland Proteome: Insights into Bothrops jararaca Intraspecific Venom Variation. J Proteome Res, 15(10):3752-3762(2016) [pubmed]

curator keyword: Biological

submitter keyword: snake venom proteome, venom gland proteome, venom variability, Bothrops jararaca

André Zelanis
contact affiliation	Functional Proteomics Laboratory - Department of Science and Technology, Federal University of Sao Paulo (UNIFESP), Sao Jose dos Campos, Sao Paulo, Brazil
contact email	andre.zelanis@unifesp.br
lab head
Andre Zelanis
contact affiliation	Laboratory of Functional Proteomics - Department of Science and Technology - Federal University of São Paulo (UNIFESP), Sao Jose dos Campos-SP, Brazil
contact email	zelanis@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004186
     1. Label: PRIDE project
     2. Name: Qualitative and quantitative analysis of Bothrops jararaca venom gland proteome