PXD004183

PXD004183 is an original dataset announced via ProteomeXchange.

Title	Identification of Plasmodium yoelii ALBA4 Binding partners by Immunoprecipitation/nano LC-MS/MS
Description	RNA-binding proteins, such as DOZI/CITH and Puf2, have been shown to play critical roles in the life cycle in Plasmodium species. One of the characterized functions of these RNA-binding proteins is to bind to mRNAs and regulate their fates, and it is hypothesized that this regulation of mRNA homeostasis of specific transcripts is important for successful infection of both vectors and hosts by these parasites. To further understand the role that other RNA-binding proteins may play in translational repression and other critical processes in the parasite, we investigated ALBA4, another RNA-binding protein implicated in translational repression. We appended a C-terminal GFP-tag to ALBA4 (ALBA4::GFP), and then performed immunoprecipitations on chemically cross-linked samples using Streptavidin-coated beads coated with a biotin-conjugated anti-GFP antibody to capture the ALBA4 complex(es). We performed these experiments in multiple life cycle stages, including sexual stages (gametocytes) and asexual stage (schizonts). In gametocytes, we determined that ALBA4 associates with translational repression machinery. This is also the case in schizonts, however ALBA4 also associates with complexes involved in active translation, mRNA export, and mRNA degradation. We hypothesize that ALBA4 plays a multi-faceted role in mRNA homeostasis by associating with multiple protein complexes in a stage-specific manner.
HostingRepository	PRIDE
AnnounceDate	2017-08-28
AnnouncementXML	Submission_2017-08-28_02:22:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Lindner
SpeciesList	scientific name: Plasmodium yoelii; NCBI TaxID: 5861;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-19 01:30:37	ID requested
⏵ 1	2017-08-28 02:22:38	announced

Mu, ñ, oz EE, Hart KJ, Walker MP, Kennedy MF, Shipley MM, Lindner SE, ALBA4 modulates its stage-specific interactions and specific mRNA fates during Plasmodium yoelii growth and transmission. Mol Microbiol, 106(2):266-284(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: RNA-binding protein, ALBA4, Plasmodium, gametocytes

Scott Eugene Lindner
contact affiliation	Biochemistry and Molecular Biology Department, Eberly College of Science, Pennsylvania State University, University Park, PA, USA.
contact email	Scott.Lindner@psu.edu
lab head
Scott Lindner
contact affiliation	Penn State University
contact email	Scott.Lindner@psu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004183
     1. Label: PRIDE project
     2. Name: Identification of Plasmodium yoelii ALBA4 Binding partners by Immunoprecipitation/nano LC-MS/MS