PXD004174

PXD004174 is an original dataset announced via ProteomeXchange.

Title	Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using CE-ESI-HRMS
Description	In this work, we integrate capillary electrophoresis electrospray ionization mass spectrometry, bottom-up proteomics, and single-cell microanalysis to enable the label-free quantification (LFQ) of proteins in single embryonic cells (D11 blastomeres) in the 16-cell frog (Xenopus laevis) embryo. Based on the LFQ data, we find translational differences between the blastomeres even within the same cell type.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:50.142.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camille Lombard-Banek
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-18 02:30:24	ID requested
1	2016-06-23 02:15:02	announced
⏵ 2	2024-10-22 04:29:51	announced	2024-10-22: Updated project metadata.

Lombard-Banek C, Reddy S, Moody SA, Nemes P, Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS). Mol Cell Proteomics, 15(8):2756-68(2016) [pubmed]

curator keyword: Biological

submitter keyword: blastomere, embryo, proteomics, Xenopus, mass spectrometry, Fusion, label free quantification,Single cell, LFQ, capillary electrophoresis, electrospray ionization, Impact HD

Peter Nemes
contact affiliation	The George Washington University
contact email	petern@gwu.edu
lab head
Camille Lombard-Banek
contact affiliation	The George Washington University
contact email	clombard@gwu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004174

PRIDE project URI

• PRIDE
  1. PXD004174
     1. Label: PRIDE project
     2. Name: Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using CE-ESI-HRMS