PXD004163

PXD004163 is an original dataset announced via ProteomeXchange.

Title	Proteomics of U1810 cells upon treatment with microRNAs with an AAGUGC seed motif.
Description	microRNA dysregulation is a common feature of cancer cells, but the complex roles of microRNAs in cancer are not fully elucidated. Here we used functional genomics to identify oncogenic microRNAs in non-small cell lung cancer and to evaluate their impact on response to EGFR targeting therapy. Our data demonstrate that microRNAs with an AAGUGC-motif in their seed-sequence increase both cancer cell proliferation and sensitivity to EGFR inhibitors. Global transcriptomics, proteomics and target prediction resulted in the identification of several tumor suppressors involved in the G1/S transition as targets of AAGUGC-microRNAs. The clinical implications of our findings were evaluated by analysis of public domain data supporting the link between this microRNA seed-family, their tumor suppressor targets and cancer cell proliferation. In conclusion we propose that AAGUGC-microRNAs are an integral part of an oncogenic signaling network, and that these findings have potential therapeutic implications, especially in selecting patients for EGFR-targeting therapy.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_01:12:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-17 01:53:52	ID requested
1	2016-06-28 05:22:52	announced
2	2016-11-21 04:13:09	announced	Updated publication reference for PubMed record(s): 27477696.
⏵ 3	2017-10-24 01:12:08	announced	Updated project metadata.

Zhou Y, Frings O, Branca RM, Boekel J, le Sage C, Fredlund E, Agami R, Orre LM, microRNAs with AAGUGC seed motif constitute an integral part of an oncogenic signaling network. Oncogene, 36(6):731-745(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, U1810, microRNA, miRNA, HiRIEF, LC-MS

Janne Lehtiö
contact affiliation	Dept. Oncology Pathology, Karolinska Institutet, and Scilifelab, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
Rui Branca
contact affiliation	Clinical Proteomics Unit, Dep. of Oncology-Pathology
contact email	rui.mamede-branca@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004163

PRIDE project URI

• PRIDE
  1. PXD004163
     1. Label: PRIDE project
     2. Name: Proteomics of U1810 cells upon treatment with microRNAs with an AAGUGC seed motif.