PXD004158

PXD004158 is an original dataset announced via ProteomeXchange.

Title	Interaction between bovine IL-1-stimulated cartilage and human serum, LC-MS/MS
Description	Joint diseases are often characterized by inflammatory processes resulting in pathological changes in joint tissues, including cartilage degradation and release of components to the synovial fluid. The complement system plays a central role in promoting the inflammation. Since several cartilage proteins are known to interact with complement, causing either activation or inhibition of the system, we aimed to investigate these interactions comprehensively. Bovine cartilage explants were cultured with interleukin-1alpha (IL-1a) to induce cartilage degradation, followed by incubation with human serum to allow interactions with complement. Label-free selected reaction monitoring (SRM) mass spectrometry (MS) was then used to specifically quantify complement proteins interacting with the cartilage explant. In parallel, the time-dependent degradation of cartilage was detected using tandem MS (MS/MS). Complement proteins resulting from activation of the classical and alternative pathway as well as the terminal pathway were detected on IL-1a stimulated cartilage at time points when clear alterations in the extracellular matrix composition had occurred. To confirm SRM results indicating complement activation, increased levels of the complement activation product C4d were detected by ELISA in serum after incubation with IL-1a stimulated cartilage. Further, typical activated (cleaved) C3 fragments were detected by western blotting of urea extracts of IL-1a stimulated cartilage. No complement activation was triggered by cartilage cultured in the absence of IL-1a. Components released from IL-1a stimulated cartilage during culture had an inhibitory effect on complement activation. These were released after a longer incubation period with IL-1a and may represent a feedback reaction to cartilage-triggered complement activation observed after a shorter incubation period.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:15.382.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camilla Melin Fürst
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-16 03:50:48	ID requested
1	2016-09-27 08:08:43	announced
⏵ 2	2024-10-22 04:30:16	announced	2024-10-22: Updated project metadata.

Melin F, ü, rst C, Å, hrman E, Bratteby K, Waldemarson S, Malmstr, ö, m J, Blom AM, Quantitative Mass Spectrometry To Study Inflammatory Cartilage Degradation and Resulting Interactions with the Complement System. J Immunol, 197(8):3415-3424(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: IL-1, bovine cartilage,human serum, LC-MS/MS

Anna Maria Blom
contact affiliation	Division of Medical Protein Chemistry, Department of Translational Medicine, Lund University, Sweden
contact email	anna.blom@med.lu.se
lab head
Camilla Melin Fürst
contact affiliation	Lund University
contact email	camilla.melin_furst@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004158
     1. Label: PRIDE project
     2. Name: Interaction between bovine IL-1-stimulated cartilage and human serum, LC-MS/MS