PXD004152

PXD004152 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis reveals the effects of human neural stem cells on multiple brain regions
Description	Parkinson’s disease (PD) is one of the most common neurodegenerative disease caused by diminution of neurons in the substantia nigra (SN) which projects dopaminergic (DA) axons to the striatum and other target areas. Recently, accumulating data demonstrated the prospects of cell replacement therapy by using neural stem cell (NSCs) transplantation. However the mechanisms underlying the potential efficacy are not fully understood. To gain new insights into the mechanisms of 6-OHDA induced lesion and potential efficacy of hNSCs transplantation, Intrastriatal 6-Hydroxydopamine (6-OHDA) injected parkinsonian mice were unilaterally engrafted with undifferentiated human NSCs to striatum (ST). High-throughput quantitative proteomic approach was utilized to characterize the proteome profiles of PD related brain regions in these mice including SN, ST, olfactory bulb (OB) and subventricular zone (SVZ). The abundance of more than 5000 proteins with high confidence in each region was determined in this study which represents the most extensive proteomic study of PD mouse models to date. In addition to the disruption of the DA system, the quantitative analysis demonstrated the profound disturbance of SVZ proteome after 6-OHDA insult, in which the abundance of more than 20% proteins was significantly changed. After hNSCs engraftment, the proteome of SVZ was restored and the astrocytes in ST was greatly activated in companion with the increase in neurotrophic factors. Furthermore, bioinformatics analysis demonstrated that changes in proteome was not caused by the proliferation of hNSCs or their progeny, but rather by the reaction of endogenous cells. Overall, this study reveals that hNSCs benefits parkinsonian animals by eliciting endogenous responses in multiple brain regions, and discovers the unexpected role of SVZ cells in PD progress and treatment, providing new therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2017-03-29
AnnouncementXML	Submission_2017-03-29_00:50:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-16 01:41:56	ID requested
⏵ 1	2017-03-29 00:50:21	announced

Zuo F, Xiong F, Wang X, Li X, Wang R, Ge W, Bao X, Intrastriatal Transplantation of Human Neural Stem Cells Restores the Impaired Subventricular Zone in Parkinsonian Mice. Stem Cells, 35(6):1519-1531(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Parkinson's disease, hNSCs transplantation, Proteomics, Subventricular zone

Wei Ge
contact affiliation	Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College; Dongdansantiao 5, Beijing, China.
contact email	Wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004152
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis reveals the effects of human neural stem cells on multiple brain regions