PXD004150

PXD004150 is an original dataset announced via ProteomeXchange.

Title	protein turnover during homeostatic plasticity
Description	Using quantitative proteomics we identified group of synaptic genes with decreased protein synthesis during homeostatic plasticity. To obtain further information about their mRNA levels/sequences (3’UTR) we performed polyA RNAseq. Using EISA analysis of Ribominus RNAseq dataset we could further differentiate between transcriptional respective post-transcriptional dependent alterations in mRNA levels. In addition Ribominus RNAseq from cell body and processes (dendrites, axons) RNA samples showed us local changes in mRNA levels during homeostatic plasticity. At the end, small RNAseq helped us to identify miRNAs that are increased during homeostatic plasticity and might regulate downregulated genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:00.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Rajman
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-16 01:26:19	ID requested
1	2017-04-05 05:01:08	announced
2	2017-05-15 00:46:32	announced	Updated publication reference for PubMed record(s): 28487411.
⏵ 3	2024-10-22 04:30:04	announced	2024-10-22: Updated project metadata.

Rajman M, Metge F, Fiore R, Khudayberdiev S, Aksoy-Aksel A, Bicker S, Ruedell Reschke C, Raoof R, Brennan GP, Delanty N, Farrell MA, O'Brien DF, Bauer S, Norwood B, Veno MT, Kr, ü, ger M, Braun T, Kjems J, Rosenow F, Henshall DC, Dieterich C, Schratt G, A microRNA-129-5p/Rbfox crosstalk coordinates homeostatic downscaling of excitatory synapses. EMBO J, 36(12):1770-1787(2017) [pubmed]

10.15252/embj.201695748;

curator keyword: Biological

submitter keyword: neuron SILAC homeostatic plasticity

Gerhard Schratt
contact affiliation	Marburg University
contact email	schratt@staff.uni-marburg.de
lab head
Marek Rajman
contact affiliation	Philipps-University Marburg
contact email	marek.rajman@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD004150

PRIDE project URI

• PRIDE
  1. PXD004150
     1. Label: PRIDE project
     2. Name: protein turnover during homeostatic plasticity