PXD004145

PXD004145 is an original dataset announced via ProteomeXchange.

Title	Adaptation to pH and pH signaling in the phytopathogenic fungus Botrytis cinerea
Description	The grey mould fungus Botrytis cinerea is capable of developing on a wide variety of host plants and different organs that differ greatly in their pH values. The strategy developed by this necrotrophic fungus is partly based on the acidification of the plant tissues via the secretion of several organic acids including oxalic acid. That’s why B.cinerea is considered as an “acidic” fungus. However, we have previously shown that it is also capable of alkalinizing its ambient environment during sunflower cotyledons or bean leaves infection and that the alkalinization is achieved by amino acids catabolism and ammonia secretion ( Billon-grand et al, 2012). This capacity to modulate the surrounding pH reveals also an ability to regulate the expression and the secretion of an arsenal of enzymes in order to produce virulence factors in accordance with the ambient pH and enzymes requirement for optimal activity. Expression of pH-regulated genes is controlled by the highly conserved signaling pathway Pal/Pac that leads to activation of the zinc finger transcription factor PACC under neutral or alkaline conditions. Investigations of the role of this pH regulator in the infectious process of B.cinerea are presented.
HostingRepository	PRIDE
AnnounceDate	2021-09-13
AnnouncementXML	Submission_2021-09-13_01:41:54.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea); NCBI TaxID: 332648;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-13 03:37:20	ID requested
⏵ 1	2021-09-13 01:41:55	announced

Rascle C, Dieryckx C, Dupuy JW, Muszkieta L, Souibgui E, Droux M, Bruel C, Girard V, Poussereau N, The pH regulator PacC: a host-dependent virulence factor in Botrytis cinerea. Environ Microbiol Rep, 10(5):555-568(2018) [pubmed]

curator keyword: Biological

submitter keyword: Botrytis cinerea, pH-regulated genes, LC-MS, Quantitative proteomics,

Vincent Girard
contact affiliation	Laboratoire Mixte UMR 5240 Centre National de la Recherche Scientifique /INSA/UCBL/Bayer CropScience, Plateforme de protéomique, Lyon, France.
contact email	vincent.girard@bayer.com
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD004145

PRIDE project URI

• PRIDE
  1. PXD004145
     1. Label: PRIDE project
     2. Name: Adaptation to pH and pH signaling in the phytopathogenic fungus Botrytis cinerea