PXD004142 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human oocyte proteome and secretome |
Description | The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per cell, we consistently identified ~450 proteins from individual oocytes. When comparing proteomes of individual oocytes at the germinal vesicle (GV) and metaphase II (MII) stage by label-free quantification, we found several proteins preferentially expressed in matuare and immature cells. |
HostingRepository | PRIDE |
AnnounceDate | 2016-06-01 |
AnnouncementXML | Submission_2016-06-01_01:09:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sophia Foehr |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-12 08:18:34 | ID requested | |
⏵ 1 | 2016-06-01 01:09:39 | announced | |
Publication List
Virant-Klun I, Leicht S, Hughes C, Krijgsveld J, Identification of Maturation-Specific Proteins by Single-Cell Proteomics of Human Oocytes. Mol Cell Proteomics, 15(8):2616-27(2016) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: Human oocytes |
Contact List
Jeroen Krijgsveld |
contact affiliation | German Cancer Research Center, Heidelberg, Germany |
contact email | j.krijgsveld@dkfz.de |
lab head | |
Sophia Foehr |
contact affiliation | EMBL |
contact email | foehr@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004142 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004142
- Label: PRIDE project
- Name: Human oocyte proteome and secretome