PXD004133

PXD004133 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium tuberculosis protein production pipeline
Description	Purpose: Tuberculosis is still a major threat to global health. New tools and strategies to produce disease-related proteins are quintessential for the development of novel vaccines and diagnostic markers. Experimental design: To obtain recombinant proteins from Mycobacterium tuberculosis (Mtb) for use in clinical applications, a standardized procedure was developed that includes subcloning, protein expression in Mycobacterium smegmatis and protein purification using chromatography. The potential for the different protein targets to serve as diagnostic markers for tuberculosis was established using multiplex immunoassays. Results: Twelve soluble proteins from Mtb, including one protein complex, were purified to homogeneity following recombinant expression in M. smegmatis. Protein purity was assessed both by size exclusion chromatography and mass spectrometry. Multiplex serological testing of the final protein preparations showed that all but one protein displayed a clear antibody response in serum samples from 278 tuberculosis patients. Conclusion and clinical relevance: The established workflow comprises a simple, cost-effective and scalable pipeline for production of soluble proteins from Mtb and can be used to prioritize immunogenic proteins suitable for use as diagnostic markers.
HostingRepository	PRIDE
AnnounceDate	2018-06-27
AnnouncementXML	Submission_2018-06-27_03:51:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joanna Kirkpatrick
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-11 05:45:54	ID requested
⏵ 1	2018-06-27 03:51:30	announced

Milewski MC, Broger T, Kirkpatrick J, Filomena A, Komadina D, Schneiderhan-Marra N, Wilmanns M, Parret AH, A standardized production pipeline for high profile targets from Mycobacterium tuberculosis. Proteomics Clin Appl, 10(9-10):1049-1057(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium tuberculosis, protein production, diagnostic marker

Annabel Parret
contact affiliation	Head of the Molecular Biology and Biochemistry Laboratory EMBL Hamburg Notkestrasse 85, 22607 Hamburg, Germany
contact email	parret@embl-hamburg.de
lab head
Joanna Kirkpatrick
contact affiliation	Leibniz Institute on Aging - FLI
contact email	joanna.kirkpatrick@leibniz-fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004133
     1. Label: PRIDE project
     2. Name: Mycobacterium tuberculosis protein production pipeline