PXD004125

PXD004125 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants
Description	We acquired quantitative proteomics data from eight week old ppi2 and tic56-1 mutants as well as from wild type treated with 60 µg/ml spectinomycin and related them to their respective wildtype reference. Protein identification and quantification was performed by data-independent MSE acquisition on a Synapt G2S mass spectrometer (Waters). Quantification was based on the Hi3 method (Silva et al., 2006). Silva, J.C., Gorenstein, M.V., Li, G.Z., Vissers, J.P., and Geromanos, S.J. (2006). Absolute quantification of proteins by LCMSE: a virtue of parallel MS acquisition. Molecular & cellular proteomics : MCP 5, 144-156.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:09.288.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sacha Baginsky
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-11 00:07:11	ID requested
1	2017-01-02 02:44:50	announced
2	2017-10-24 02:47:42	announced	Updated project metadata.
⏵ 3	2024-10-22 04:30:16	announced	2024-10-22: Updated project metadata.

K, ö, hler D, Helm S, Agne B, Baginsky S, Importance of Translocon Subunit Tic56 for rRNA Processing and Chloroplast Ribosome Assembly. Plant Physiol, 172(4):2429-2444(2016) [pubmed]

curator keyword: Biological

submitter keyword: HDMSE, tic56-1, plastid, spectinomycin, plastidic translation, quantitative mass spectrometry,Shotgun proteomics, TOC-TIC-import machinery, Hi3 quantification, ppi2

Sacha Baginsky
contact affiliation	Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany
contact email	sacha.baginsky@biochemtech.uni-halle.de
lab head
Sacha Baginsky
contact affiliation	Institut fuer Biochemie und Biotechnologie
contact email	pflanzen@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004125
     1. Label: PRIDE project
     2. Name: Mass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants