PXD004125 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants |
Description | We acquired quantitative proteomics data from eight week old ppi2 and tic56-1 mutants as well as from wild type treated with 60 µg/ml spectinomycin and related them to their respective wildtype reference. Protein identification and quantification was performed by data-independent MSE acquisition on a Synapt G2S mass spectrometer (Waters). Quantification was based on the Hi3 method (Silva et al., 2006). Silva, J.C., Gorenstein, M.V., Li, G.Z., Vissers, J.P., and Geromanos, S.J. (2006). Absolute quantification of proteins by LCMSE: a virtue of parallel MS acquisition. Molecular & cellular proteomics : MCP 5, 144-156. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:30:09.288.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sacha Baginsky |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-11 00:07:11 | ID requested | |
1 | 2017-01-02 02:44:50 | announced | |
2 | 2017-10-24 02:47:42 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:30:16 | announced | 2024-10-22: Updated project metadata. |
Publication List
K, ö, hler D, Helm S, Agne B, Baginsky S, Importance of Translocon Subunit Tic56 for rRNA Processing and Chloroplast Ribosome Assembly. Plant Physiol, 172(4):2429-2444(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: HDMSE, tic56-1, plastid, spectinomycin, plastidic translation, quantitative mass spectrometry,Shotgun proteomics, TOC-TIC-import machinery, Hi3 quantification, ppi2 |
Contact List
Sacha Baginsky |
contact affiliation | Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany |
contact email | sacha.baginsky@biochemtech.uni-halle.de |
lab head | |
Sacha Baginsky |
contact affiliation | Institut fuer Biochemie und Biotechnologie |
contact email | pflanzen@biochemtech.uni-halle.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004125
- Label: PRIDE project
- Name: Mass spectrometry based quantitative proteome analyses for the import mutants ppi2 and tic56-1 as well as for spectinomycin treated Arabidopsis thalian plants