PXD004123

PXD004123 is an original dataset announced via ProteomeXchange.

Title	Label-Free Quantitative Analysis of the Salivary Pellicle’s Proteome
Description	The salivary pellicle, covering natural as well as restored tooth surfaces in the oral cavity as an immobilized protein-rich layer, acts as an important biological mediator at the tooth-saliva-interface. Going beyond previous non-quantitative analyses on pooled samples, we here analyzed the pellicle’s proteome of four individuals, separately on three consecutive days, and determined the relative protein abundance by a label-free quantitative nano-LC-MS/MS approach. We identified 72 major proteins in the initial pellicles formed after 3 min intraorally on ceramic specimens with high inter-individual and inter-day consistency, while significant differences in protein abundance were evident between subjects exhibiting unique individual pellicle profiles. Furthermore, we compared the relative protein abundance with corresponding saliva samples of the same individuals and provide first data on significantly enriched and depleted salivary proteins (p < 0.05) within the salivary pellicle. Our findings reveal the initial adsorption of salivary proteins to solid substrates to be a rapid, highly selective, and reproducible process leading to the immobilization of a broad range of protective proteins and enzymes on the substratum surface within a few minutes. This provides evidence that the pellicle layer might be physiologically functional even without further maturation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:36.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Médard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-10 06:50:08	ID requested
1	2022-03-01 09:35:59	announced
⏵ 2	2024-10-22 05:34:37	announced	2024-10-22: Updated project metadata.

10.1016/j.colsurfb.2017.01.005;

Delius J, Trautmann S, M, é, dard G, Kuster B, Hannig M, Hofmann T, Label-free quantitative proteome analysis of the surface-bound salivary pellicle. Colloids Surf B Biointerfaces, 152():68-76(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Orbitrap Velos, saliva, acquired pellicle,human

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics Technical University of Munich
contact email	kuster@tum.de
lab head
Guillaume Médard
contact affiliation	Technical University of Munich
contact email	g.medard@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD004123

PRIDE project URI

• PRIDE
  1. PXD004123
     1. Label: PRIDE project
     2. Name: Label-Free Quantitative Analysis of the Salivary Pellicle’s Proteome