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PXD004123

PXD004123 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-Free Quantitative Analysis of the Salivary Pellicle’s Proteome
DescriptionThe salivary pellicle, covering natural as well as restored tooth surfaces in the oral cavity as an immobilized protein-rich layer, acts as an important biological mediator at the tooth-saliva-interface. Going beyond previous non-quantitative analyses on pooled samples, we here analyzed the pellicle’s proteome of four individuals, separately on three consecutive days, and determined the relative protein abundance by a label-free quantitative nano-LC-MS/MS approach. We identified 72 major proteins in the initial pellicles formed after 3 min intraorally on ceramic specimens with high inter-individual and inter-day consistency, while significant differences in protein abundance were evident between subjects exhibiting unique individual pellicle profiles. Furthermore, we compared the relative protein abundance with corresponding saliva samples of the same individuals and provide first data on significantly enriched and depleted salivary proteins (p < 0.05) within the salivary pellicle. Our findings reveal the initial adsorption of salivary proteins to solid substrates to be a rapid, highly selective, and reproducible process leading to the immobilization of a broad range of protective proteins and enzymes on the substratum surface within a few minutes. This provides evidence that the pellicle layer might be physiologically functional even without further maturation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:34:36.737.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume Médard
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-10 06:50:08ID requested
12022-03-01 09:35:59announced
22024-10-22 05:34:37announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.colsurfb.2017.01.005;
Delius J, Trautmann S, M, é, dard G, Kuster B, Hannig M, Hofmann T, Label-free quantitative proteome analysis of the surface-bound salivary pellicle. Colloids Surf B Biointerfaces, 152():68-76(2017) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Orbitrap Velos, saliva, acquired pellicle,human
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics Technical University of Munich
contact emailkuster@tum.de
lab head
Guillaume Médard
contact affiliationTechnical University of Munich
contact emailg.medard@tum.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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