PXD004123 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-Free Quantitative Analysis of the Salivary Pellicle’s Proteome |
Description | The salivary pellicle, covering natural as well as restored tooth surfaces in the oral cavity as an immobilized protein-rich layer, acts as an important biological mediator at the tooth-saliva-interface. Going beyond previous non-quantitative analyses on pooled samples, we here analyzed the pellicle’s proteome of four individuals, separately on three consecutive days, and determined the relative protein abundance by a label-free quantitative nano-LC-MS/MS approach. We identified 72 major proteins in the initial pellicles formed after 3 min intraorally on ceramic specimens with high inter-individual and inter-day consistency, while significant differences in protein abundance were evident between subjects exhibiting unique individual pellicle profiles. Furthermore, we compared the relative protein abundance with corresponding saliva samples of the same individuals and provide first data on significantly enriched and depleted salivary proteins (p < 0.05) within the salivary pellicle. Our findings reveal the initial adsorption of salivary proteins to solid substrates to be a rapid, highly selective, and reproducible process leading to the immobilization of a broad range of protective proteins and enzymes on the substratum surface within a few minutes. This provides evidence that the pellicle layer might be physiologically functional even without further maturation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:34:36.737.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guillaume Médard |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-10 06:50:08 | ID requested | |
1 | 2022-03-01 09:35:59 | announced | |
⏵ 2 | 2024-10-22 05:34:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.colsurfb.2017.01.005; |
Delius J, Trautmann S, M, é, dard G, Kuster B, Hannig M, Hofmann T, Label-free quantitative proteome analysis of the surface-bound salivary pellicle. Colloids Surf B Biointerfaces, 152():68-76(2017) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Orbitrap Velos, saliva, acquired pellicle,human |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics Technical University of Munich |
contact email | kuster@tum.de |
lab head | |
Guillaume Médard |
contact affiliation | Technical University of Munich |
contact email | g.medard@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004123
- Label: PRIDE project
- Name: Label-Free Quantitative Analysis of the Salivary Pellicle’s Proteome