PXD004120

PXD004120 is an original dataset announced via ProteomeXchange.

Title	Pre-assembled Nuclear Pores Insert into the Nuclear Envelope During Early Development
Description	Nuclear Pore Complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes and early embryos, NPCs not only reside within the NE but also at some endoplasmatic reticulum (ER) membrane sheets, termed annulate lamellae (AL). Although a role for AL as NPC storage pools has been discussed, it remains controversial if and how they contribute to the NPC density at the NE. Here we show that AL insert into the NE as the ER feeds rapid nuclear expansion in Drosophila blastoderm embryos. We demonstrate that NPCs within AL resemble pore scaffolds that mature only upon insertion into the NE. We delineate a topological model in which NE openings are critical for AL uptake that nevertheless occurs without compromising the permeability barrier of the NE. We finally show that this unanticipated mode of pore insertion is developmentally regulated and operates prior to gastrulation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:11.738.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie-Therese Mackmull
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-10 00:26:27	ID requested
1	2016-07-12 03:21:08	announced
⏵ 2	2024-10-22 04:30:12	announced	2024-10-22: Updated project metadata.

10.1016/j.cell.2016.06.015;

Hampoelz B, Mackmull MT, Machado P, Ronchi P, Bui KH, Schieber N, Santarella-Mellwig R, Necakov A, Andr, é, s-Pons A, Philippe JM, Lecuit T, Schwab Y, Beck M, Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development. Cell, 166(3):664-678(2016) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS, subcellular fractionation, Drosophila melanogaster

Martin Beck
contact affiliation	Structural and Computational Biology Unit European Molecular Biology Laboratory (EMBL) Meyerhofstr. 1 69117 Heidelberg Germany
contact email	martin.beck@embl.de
lab head
Marie-Therese Mackmull
contact affiliation	EMBL
contact email	marie.mackmull@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD004120

PRIDE project URI

• PRIDE
  1. PXD004120
     1. Label: PRIDE project
     2. Name: Pre-assembled Nuclear Pores Insert into the Nuclear Envelope During Early Development