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PXD004120

PXD004120 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePre-assembled Nuclear Pores Insert into the Nuclear Envelope During Early Development
DescriptionNuclear Pore Complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes and early embryos, NPCs not only reside within the NE but also at some endoplasmatic reticulum (ER) membrane sheets, termed annulate lamellae (AL). Although a role for AL as NPC storage pools has been discussed, it remains controversial if and how they contribute to the NPC density at the NE. Here we show that AL insert into the NE as the ER feeds rapid nuclear expansion in Drosophila blastoderm embryos. We demonstrate that NPCs within AL resemble pore scaffolds that mature only upon insertion into the NE. We delineate a topological model in which NE openings are critical for AL uptake that nevertheless occurs without compromising the permeability barrier of the NE. We finally show that this unanticipated mode of pore insertion is developmentally regulated and operates prior to gastrulation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:30:11.738.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarie-Therese Mackmull
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-10 00:26:27ID requested
12016-07-12 03:21:08announced
22024-10-22 04:30:12announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.cell.2016.06.015;
Hampoelz B, Mackmull MT, Machado P, Ronchi P, Bui KH, Schieber N, Santarella-Mellwig R, Necakov A, Andr, é, s-Pons A, Philippe JM, Lecuit T, Schwab Y, Beck M, Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development. Cell, 166(3):664-678(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: LC-MS/MS, subcellular fractionation, Drosophila melanogaster
Contact List
Martin Beck
contact affiliationStructural and Computational Biology Unit European Molecular Biology Laboratory (EMBL) Meyerhofstr. 1 69117 Heidelberg Germany
contact emailmartin.beck@embl.de
lab head
Marie-Therese Mackmull
contact affiliationEMBL
contact emailmarie.mackmull@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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