PXD004120 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pre-assembled Nuclear Pores Insert into the Nuclear Envelope During Early Development |
Description | Nuclear Pore Complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes and early embryos, NPCs not only reside within the NE but also at some endoplasmatic reticulum (ER) membrane sheets, termed annulate lamellae (AL). Although a role for AL as NPC storage pools has been discussed, it remains controversial if and how they contribute to the NPC density at the NE. Here we show that AL insert into the NE as the ER feeds rapid nuclear expansion in Drosophila blastoderm embryos. We demonstrate that NPCs within AL resemble pore scaffolds that mature only upon insertion into the NE. We delineate a topological model in which NE openings are critical for AL uptake that nevertheless occurs without compromising the permeability barrier of the NE. We finally show that this unanticipated mode of pore insertion is developmentally regulated and operates prior to gastrulation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:30:11.738.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie-Therese Mackmull |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-10 00:26:27 | ID requested | |
1 | 2016-07-12 03:21:08 | announced | |
⏵ 2 | 2024-10-22 04:30:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.cell.2016.06.015; |
Hampoelz B, Mackmull MT, Machado P, Ronchi P, Bui KH, Schieber N, Santarella-Mellwig R, Necakov A, Andr, é, s-Pons A, Philippe JM, Lecuit T, Schwab Y, Beck M, Pre-assembled Nuclear Pores Insert into the Nuclear Envelope during Early Development. Cell, 166(3):664-678(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: LC-MS/MS, subcellular fractionation, Drosophila melanogaster |
Contact List
Martin Beck |
contact affiliation | Structural and Computational Biology Unit European Molecular Biology Laboratory (EMBL) Meyerhofstr. 1 69117 Heidelberg Germany |
contact email | martin.beck@embl.de |
lab head | |
Marie-Therese Mackmull |
contact affiliation | EMBL |
contact email | marie.mackmull@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004120
- Label: PRIDE project
- Name: Pre-assembled Nuclear Pores Insert into the Nuclear Envelope During Early Development