PXD004113

PXD004113 is an original dataset announced via ProteomeXchange.

Title	Proteome and Secretome Analysis Reveals Differential Post-Transcriptional Regulation of Toll-Like Receptor Responses
Description	Toll-like receptors (TLRs) are essential sensors in innate immunity and among the first to detect invading pathogens. Many studies of TLR responses have focused on the intracellular signaling events that occur upon receptor stimulation. However, proteins released from the cell play a key role in cell-cell communication during an immune response. We have used mass spectrometry-based proteomic methods to investigate both the intracellular and extracellular responses of macrophages stimulated with individual TLR2, TLR4, and TLR7 ligands and whole pathogens. We performed global quantitative analyses of the mouse macrophage proteome and secretome using stable isotope labeling with amino acids and high-resolution mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-03-06
AnnouncementXML	Submission_2017-03-06_06:58:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-09 01:28:04	ID requested
⏵ 1	2017-03-06 06:58:05	announced

Koppenol-Raab M, Sjoelund V, Manes NP, Gottschalk RA, Dutta B, Benet ZL, Fraser ID, Nita-Lazar A, Proteome and Secretome Analysis Reveals Differential Post-transcriptional Regulation of Toll-like Receptor Responses. Mol Cell Proteomics, 16(4 suppl 1):S172-S186(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, Macrophage, RAW264.7, Secretome, TLR, LC-MSMS, 3plex SILAC, Targeted MS

Aleksandra Nita-Lazar
contact affiliation	National Institutes of Health, Bethesda, MD, USA
contact email	nitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004113
     1. Label: PRIDE project
     2. Name: Proteome and Secretome Analysis Reveals Differential Post-Transcriptional Regulation of Toll-Like Receptor Responses