PXD004108

PXD004108 is an original dataset announced via ProteomeXchange.

Title	Mouse liver regeneration quantitative proteomics
Description	The liver is the only organ in mammals, which fully regenerates after injury. To identify novel regulators of liver regeneration, we performed quantitative large-scale proteomics analysis of subcellular fractions from normal versus regenerating mouse liver. Proteins of the ubiquitin-proteasome pathway were rapidly regulated by partial hepatectomy, with the ubiquitin ligase Nedd4-1 being among the top hits. Knock-down of Nedd4-1 in hepatocytes in vivo through nanoparticle-mediated delivery of siRNA caused severe liver damage after partial hepatectomy and impaired regeneration, resulting in liver failure. Mechanistically, we demonstrate that Nedd4-1 is required for efficient activation of Erk1/2 signaling by receptor tyrosine kinases involved in liver regeneration through inhibition of receptor internalization, thus controlling a major pro-mitogenic and cytoprotective signaling pathway in the regenerating liver. These results highlight the power of large-scale proteomics to identify key players in liver regeneration and the importance of posttranslational regulation of growth factor signaling in this process.
HostingRepository	PRIDE
AnnounceDate	2017-09-13
AnnouncementXML	Submission_2017-09-13_00:36:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich auf dem Keller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-06 00:52:07	ID requested
⏵ 1	2017-09-13 00:36:57	announced

Bachofner M, Speicher T, Bogorad RL, Muzumdar S, Derrer CP, H, ü, rlimann F, B, ö, hm F, Nanni P, Kockmann T, Kachaylo E, Meyer M, Padrissa-Alt, é, s S, Graf R, Anderson DG, Koteliansky V, Auf dem Keller U, Werner S, Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration. Dev Cell, 42(6):616-625.e8(2017) [pubmed]

curator keyword: Biological

submitter keyword: mouse, liver regeneration, partial hepatectomy, iTRAQ, DIA

Sabine Werner
contact affiliation	ETH Zurich
contact email	sabine.werner@biol.ethz.ch
lab head
Ulrich auf dem Keller
contact affiliation	Department of Biology
contact email	ulrich.aufdemkeller@biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD004108

PRIDE project URI

• PRIDE
  1. PXD004108
     1. Label: PRIDE project
     2. Name: Mouse liver regeneration quantitative proteomics