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PXD004108

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-09-13
  • AnnouncementXML: Submission_2017-09-13_00:36:56.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Ulrich auf dem Keller
  • Title: Mouse liver regeneration quantitative proteomics
  • Description: The liver is the only organ in mammals, which fully regenerates after injury. To identify novel regulators of liver regeneration, we performed quantitative large-scale proteomics analysis of subcellular fractions from normal versus regenerating mouse liver. Proteins of the ubiquitin-proteasome pathway were rapidly regulated by partial hepatectomy, with the ubiquitin ligase Nedd4-1 being among the top hits. Knock-down of Nedd4-1 in hepatocytes in vivo through nanoparticle-mediated delivery of siRNA caused severe liver damage after partial hepatectomy and impaired regeneration, resulting in liver failure. Mechanistically, we demonstrate that Nedd4-1 is required for efficient activation of Erk1/2 signaling by receptor tyrosine kinases involved in liver regeneration through inhibition of receptor internalization, thus controlling a major pro-mitogenic and cytoprotective signaling pathway in the regenerating liver. These results highlight the power of large-scale proteomics to identify key players in liver regeneration and the importance of posttranslational regulation of growth factor signaling in this process.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
  • Instrument: LTQ Orbitrap Velos; Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-05-06 00:52:07ID requested
12017-09-13 00:36:57announced

Publication List

  1. Bachofner M, Speicher T, Bogorad RL, Muzumdar S, Derrer CP, Hürlimann F, Böhm F, Nanni P, Kockmann T, Kachaylo E, Meyer M, Padrissa-Altés S, Graf R, Anderson DG, Koteliansky V, Auf dem Keller U, Werner S, Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration. Dev Cell, ():(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: mouse, liver regeneration, partial hepatectomy, iTRAQ, DIA

Contact List

    Sabine Werner
    • contact affiliation: ETH Zurich
    • contact email: sabine.werner@biol.ethz.ch
    • lab head:
    Ulrich auf dem Keller
    • contact affiliation: Department of Biology
    • contact email: ulrich.aufdemkeller@biol.ethz.ch
    • dataset submitter:

Full Dataset Link List

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