PXD004107

PXD004107 is an original dataset announced via ProteomeXchange.

Title	A model dataset for quantitative cross-linking/mass spectrometry using isotope-labeled cross-linkers
Description	Dynamic proteins and multi-protein complexes govern most biological processes. Cross-linking/mass spectrometry (CLMS) is increasingly successful in providing residue-resolution data on static proteinaceous structures. In order to investigate the technical feasibility of recording dynamic processes using isotope-labelling for quantitation, we generated a model dataset by cross-linking human serum albumin (HSA) with the readily available cross-linker BS3-d0/d4 in different heavy/light ratios.
HostingRepository	PRIDE
AnnounceDate	2016-05-06
AnnouncementXML	Submission_2016-05-06_07:04:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-05 07:27:54	ID requested
⏵ 1	2016-05-06 07:04:18	announced

Fischer L, Chen ZA, Rappsilber J, Quantitative cross-linking/mass spectrometry using isotope-labelled cross-linkers. J Proteomics, 88():120-8(2013) [pubmed]

curator keyword: Biological, Technical

submitter keyword: Quantitative cross-linking/mass spectromentry, model dataset, HSA, isotope-labeled cross-linkers

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, UK Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Zhuo Chen
contact affiliation	University of Edinburgh
contact email	zhuo.chen@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/05/PXD004107

PRIDE project URI

• PRIDE
  1. PXD004107
     1. Label: PRIDE project
     2. Name: A model dataset for quantitative cross-linking/mass spectrometry using isotope-labeled cross-linkers