PXD004106

PXD004106 is an original dataset announced via ProteomeXchange.

Title	Soybean seed germination LC-MS/MS
Description	This study aims at addressing soybean seeds (variety Absolute RR) germination, at 48h, during optimal and salt stressed condition when treated with bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17). Soybean growth is negatively affected when exposed to 40 mM NaCl and exposure to 80 mM NaCl is often lethal. When treated with the bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17), soybean seeds (variety Absolute RR) responded positively at salt stress of up to 150 mM NaCl. Shotgun proteomics of unstressed and 100 mM NaCl stressed seeds (48 h) in combination with the LCO and Th17 revealed many known, predicted, hypothetical and unknown proteins. In all, carbon, nitrogen and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with signals. PEP carboxylase, Rubisco oxygenase large subunit, pyruvate kinase, and isocitrate lyase were some of the noteworthy proteins enhanced by the signals, along with antioxidant glutathione-S-transferase and other stress related proteins. These findings suggest that the germinating seeds alter their proteome based on bacterial signals and on stress, the specificity of this response plays a crucial role in organ maturation and transition from one stage to another in the plants life cycle; understanding this response is of fundamental importance in agriculture and, as a result, global food security.
HostingRepository	PRIDE
AnnounceDate	2016-09-28
AnnouncementXML	Submission_2016-09-28_01:07:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sowmyalakshmi Subramanian
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-05 03:37:45	ID requested
⏵ 1	2016-09-28 01:07:08	announced

Subramanian S, Ricci E, Souleimanov A, Smith DL, A Proteomic Approach to Lipo-Chitooligosaccharide and Thuricin 17 Effects on Soybean GerminationUnstressed and Salt Stress. PLoS One, 11(8):e0160660(2016) [pubmed]

curator keyword: Biological

submitter keyword: Soybean, Germination, Salt stress, Lipo-chitooligosaccharide, Thuricin17, LC-MS/MS

Donald L Smith
contact affiliation	James McGill Professor Director and CEO, BioFuelNet Canada Director, McGill Network for Innovation in Biofuels and Bioproducts Director, Eastern Canadian Oilseeds Development Initiative Plant Science Department, McGill University 21111 Lakeshore Road Ste. Anne de Bellevue, Quebec Canada H9X3V9 Phone: +1 514 398 7866 Fax: +1 514 398 7990 Website: www.mcgill.ca/plant/faculty/smith/
contact email	donald.smith@mcgill.ca
lab head
Sowmyalakshmi Subramanian
contact affiliation	McGill University
contact email	sowmyalakshmi.subramanian@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004106

PRIDE project URI

• PRIDE
  1. PXD004106
     1. Label: PRIDE project
     2. Name: Soybean seed germination LC-MS/MS