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PXD004099

PXD004099 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe CENP-T/-W complex is a binding partner of the histone chaperone FACT
DescriptionThe CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres and new deposition is absolutely required at each cell cycle for kinetochore function. However, the mechanisms underlying this new deposition of CENP-T/-W at centromeres are unclear. Here, we find that CENP-T deposition at centromeres is uncoupled from DNA synthesis. We identify Spt16 and SSRP1, subunits of the H2A-H2B histone chaperone FACT, as CENP-W binding partners through a proteomic screen. We find that the C-terminal region of Spt16 binds specifically to the histone fold region of CENP-T/-W. Furthermore, depletion of Spt16 impairs CENP-T and CENP-W deposition at endogenous centromeres and site directed targeting of Spt16 alone is sufficient to ensure local de novo CENP-T accumulation. We propose a model in which the FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres.
HostingRepositoryPRIDE
AnnounceDate2016-06-14
AnnouncementXMLSubmission_2016-06-14_03:53:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuillaume Arras
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-04 05:28:27ID requested
12016-06-14 03:53:33announced
Publication List
Prendergast L, M, ü, ller S, Liu Y, Huang H, Dingli F, Loew D, Vassias I, Patel DJ, Sullivan KF, Almouzni G, The CENP-T/-W complex is a binding partner of the histone chaperone FACT. Genes Dev, 30(11):1313-26(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: centromere, CENP, histone chaperone, mitosis
Contact List
Damarys Loew
contact affiliationLaboratoire de Spectrométrie de Masse Protéomique (LSMP) Institut Curie
contact emaildamarys.loew@curie.fr
lab head
Guillaume Arras
contact affiliationInstitut Curie
contact emailguillaume.arras@curie.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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