PXD004099

PXD004099 is an original dataset announced via ProteomeXchange.

Title	The CENP-T/-W complex is a binding partner of the histone chaperone FACT
Description	The CENP-T/-W histone fold complex, as an integral part of the inner kinetochore, is essential for building a proper kinetochore at the centromere in order to direct chromosome segregation during mitosis. Notably, CENP-T/-W is not inherited at centromeres and new deposition is absolutely required at each cell cycle for kinetochore function. However, the mechanisms underlying this new deposition of CENP-T/-W at centromeres are unclear. Here, we find that CENP-T deposition at centromeres is uncoupled from DNA synthesis. We identify Spt16 and SSRP1, subunits of the H2A-H2B histone chaperone FACT, as CENP-W binding partners through a proteomic screen. We find that the C-terminal region of Spt16 binds specifically to the histone fold region of CENP-T/-W. Furthermore, depletion of Spt16 impairs CENP-T and CENP-W deposition at endogenous centromeres and site directed targeting of Spt16 alone is sufficient to ensure local de novo CENP-T accumulation. We propose a model in which the FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres.
HostingRepository	PRIDE
AnnounceDate	2016-06-14
AnnouncementXML	Submission_2016-06-14_03:53:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Arras
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-04 05:28:27	ID requested
⏵ 1	2016-06-14 03:53:33	announced

Prendergast L, M, ü, ller S, Liu Y, Huang H, Dingli F, Loew D, Vassias I, Patel DJ, Sullivan KF, Almouzni G, The CENP-T/-W complex is a binding partner of the histone chaperone FACT. Genes Dev, 30(11):1313-26(2016) [pubmed]

curator keyword: Biological

submitter keyword: centromere, CENP, histone chaperone, mitosis

Damarys Loew
contact affiliation	Laboratoire de Spectrométrie de Masse Protéomique (LSMP) Institut Curie
contact email	damarys.loew@curie.fr
lab head
Guillaume Arras
contact affiliation	Institut Curie
contact email	guillaume.arras@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004099

PRIDE project URI

• PRIDE
  1. PXD004099
     1. Label: PRIDE project
     2. Name: The CENP-T/-W complex is a binding partner of the histone chaperone FACT