PXD004086

PXD004086 is an original dataset announced via ProteomeXchange.

Title	Characterization of the PAK4 interactome by quantitative mass spectrometry
Description	p21-activated kinase 4 (PAK4) is a serine/threonine kinase critical during development and with a proposed role in cancer and related cellular processes, including cell proliferation, survival and migration. However, while a limited number of PAK4-interacting proteins have been identified, the PAK4 interactome has not been systematically characterized. Here, we employed iTRAQ-based quantitative mass spectrometry of PAK4-immunioprecipitations from three distinct isolated cellular fractions to comprehensively profile the PAK4 interactome in human. These data will provide a valuable resource for further investigations on the role of PAK4 in physiology and disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:37.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Henrik J Johansson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-02 09:01:41	ID requested
1	2018-10-27 03:16:48	announced
⏵ 2	2024-10-22 04:28:45	announced	2024-10-22: Updated project metadata.

Zhao M, Spiess M, Johansson HJ, Olofsson H, Hu J, Lehti, ö J, Str, ö, mblad S, Identification of the PAK4 interactome reveals PAK4 phosphorylation of N-WASP and promotion of Arp2/3-dependent actin polymerization. Oncotarget, 8(44):77061-77074(2017) [pubmed]

10.18632/oncotarget.20352;

curator keyword: Biological

submitter keyword: MS/MS, pull-down, protein-protein interactions,PAK4

Janne Lehtiö
contact affiliation	Clinical proteomics mass spectrometry, SciLifeLab, Dept. oncology-pathology, Karolinska Institutet.
contact email	janne.lehtio@ki.se
lab head
Henrik J Johansson
contact affiliation	Oncology/pathology
contact email	henrik.johansson@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004086

PRIDE project URI

• PRIDE
  1. PXD004086
     1. Label: PRIDE project
     2. Name: Characterization of the PAK4 interactome by quantitative mass spectrometry