PXD004085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics on wt-MCF7 versus their LTED derivatives using dimethyl labelling |
Description | Wild type (wt) MCF7 cells, modelling breast cancer at primary diagnosis, were cultured in phenol red-free RPMI supplemented with 10% FBS and 1nM estradiol (E2). Long-term oestrogen deprived (LTED) cell lines, which model resistance to endocrine therapy, were cultured in phenol red-free RPMI in the absence of exogenous E2 and supplemented with 10% dextran charcoal-stripped bovine serum (DCC). Samples were harvested at baseline and at the point of resistance (LTED). To reveal differential protein abundances between wt-MCF7 and MCF7 LTED, the peptides were labelled with chemical labelling and underwent fractionation using OFFGEL electrophoresis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:28:48.639.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nikiana Simigdala |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-02 05:53:17 | ID requested | |
1 | 2016-06-01 01:45:01 | announced | |
⏵ 2 | 2024-10-22 04:28:49 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: MCF7, LTED,dimethyl labelling |
Contact List
Lesley-Ann Martin |
contact affiliation | The Institute of Cancer Research The Breast Cancer Now Toby Robins Research Centre |
contact email | Lesley-Ann.Martin@icr.ac.uk |
lab head | |
Nikiana Simigdala |
contact affiliation | BRFAA |
contact email | Nikiana.Simigdala@icr.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004085
- Label: PRIDE project
- Name: Quantitative proteomics on wt-MCF7 versus their LTED derivatives using dimethyl labelling