PXD004081 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction |
Description | Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we study protein distributions in bacterial biofilms using shotgun proteomics. Notably, zinc- and manganese-depleted portions of the biofilm repress the production of anti-staphylococcal molecules. Exposure to calprotectin (a host protein known to sequester metal ions at infectious foci) recapitulates responses occurring within metal-deplete portions of the biofilm and promotes interaction between P. aeruginosa and Staphylococcus aureus. Consistent with these results, the presence of calprotectin promotes co-colonization of the murine lung, and polymicrobial communities are found to co-exist in calprotectin-enriched airspaces of a cystic fibrosis lung explant. These findings, which demonstrate that metal fluctuations are a driving force of microbial community structure, have clinical implications because of the frequent occurrence of P. aeruginosa and S. aureus co-infections. |
HostingRepository | PRIDE |
AnnounceDate | 2016-06-22 |
AnnouncementXML | Submission_2016-06-22_06:30:29.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004081 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jessica Moore |
SpeciesList | scientific name: Pseudomonas aeruginosa (strain UCBPP-PA14); NCBI TaxID: 208963; |
ModificationList | Oxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-05-02 02:30:36 | ID requested | |
⏵ 1 | 2016-06-22 06:30:31 | announced | |
Publication List
Wakeman CA, Moore JL, Noto MJ, Zhang Y, Singleton MD, Prentice BM, Gilston BA, Doster RS, Gaddy JA, Chazin WJ, Caprioli RM, Skaar EP, The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction. Nat Commun, 7():11951(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Calprotectin, S100A8, S100A9, biofilms, drip flow reactor, PA14, Pseudomonas aeruginoa, |
Contact List
Richard Caprioli |
contact affiliation | Vanderbilt University, Mass Spectrometry Research Center |
contact email | Richard.m.Caprioli@Vanderbilt.edu |
lab head | |
Jessica Moore |
contact affiliation | Vanderbilt University |
contact email | jessica.l.moore.1@vanderbilt.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004081
- Label: PRIDE project
- Name: The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction