PXD004081

PXD004081 is an original dataset announced via ProteomeXchange.

Title	The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction
Description	Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we study protein distributions in bacterial biofilms using shotgun proteomics. Notably, zinc- and manganese-depleted portions of the biofilm repress the production of anti-staphylococcal molecules. Exposure to calprotectin (a host protein known to sequester metal ions at infectious foci) recapitulates responses occurring within metal-deplete portions of the biofilm and promotes interaction between P. aeruginosa and Staphylococcus aureus. Consistent with these results, the presence of calprotectin promotes co-colonization of the murine lung, and polymicrobial communities are found to co-exist in calprotectin-enriched airspaces of a cystic fibrosis lung explant. These findings, which demonstrate that metal fluctuations are a driving force of microbial community structure, have clinical implications because of the frequent occurrence of P. aeruginosa and S. aureus co-infections.
HostingRepository	PRIDE
AnnounceDate	2016-06-22
AnnouncementXML	Submission_2016-06-22_06:30:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004081
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessica Moore
SpeciesList	scientific name: Pseudomonas aeruginosa (strain UCBPP-PA14); NCBI TaxID: 208963;
ModificationList	Oxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-05-02 02:30:36	ID requested
⏵ 1	2016-06-22 06:30:31	announced

Wakeman CA, Moore JL, Noto MJ, Zhang Y, Singleton MD, Prentice BM, Gilston BA, Doster RS, Gaddy JA, Chazin WJ, Caprioli RM, Skaar EP, The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction. Nat Commun, 7():11951(2016) [pubmed]

curator keyword: Biological

submitter keyword: Calprotectin, S100A8, S100A9, biofilms, drip flow reactor, PA14, Pseudomonas aeruginoa,

Richard Caprioli
contact affiliation	Vanderbilt University, Mass Spectrometry Research Center
contact email	Richard.m.Caprioli@Vanderbilt.edu
lab head
Jessica Moore
contact affiliation	Vanderbilt University
contact email	jessica.l.moore.1@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD004081

PRIDE project URI

• PRIDE
  1. PXD004081
     1. Label: PRIDE project
     2. Name: The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction