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PXD004081

PXD004081 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction
DescriptionMicroorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we study protein distributions in bacterial biofilms using shotgun proteomics. Notably, zinc- and manganese-depleted portions of the biofilm repress the production of anti-staphylococcal molecules. Exposure to calprotectin (a host protein known to sequester metal ions at infectious foci) recapitulates responses occurring within metal-deplete portions of the biofilm and promotes interaction between P. aeruginosa and Staphylococcus aureus. Consistent with these results, the presence of calprotectin promotes co-colonization of the murine lung, and polymicrobial communities are found to co-exist in calprotectin-enriched airspaces of a cystic fibrosis lung explant. These findings, which demonstrate that metal fluctuations are a driving force of microbial community structure, have clinical implications because of the frequent occurrence of P. aeruginosa and S. aureus co-infections.
HostingRepositoryPRIDE
AnnounceDate2016-06-22
AnnouncementXMLSubmission_2016-06-22_06:30:29.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004081
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJessica Moore
SpeciesList scientific name: Pseudomonas aeruginosa (strain UCBPP-PA14); NCBI TaxID: 208963;
ModificationListOxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-05-02 02:30:36ID requested
12016-06-22 06:30:31announced
Publication List
Wakeman CA, Moore JL, Noto MJ, Zhang Y, Singleton MD, Prentice BM, Gilston BA, Doster RS, Gaddy JA, Chazin WJ, Caprioli RM, Skaar EP, The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction. Nat Commun, 7():11951(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Calprotectin, S100A8, S100A9, biofilms, drip flow reactor, PA14, Pseudomonas aeruginoa,
Contact List
Richard Caprioli
contact affiliationVanderbilt University, Mass Spectrometry Research Center
contact emailRichard.m.Caprioli@Vanderbilt.edu
lab head
Jessica Moore
contact affiliationVanderbilt University
contact emailjessica.l.moore.1@vanderbilt.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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