PXD004074

PXD004074 is an original dataset announced via ProteomeXchange.

Title	Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases
Description	Budding yeast Tsr1 is an essential ribosome biogenesis factor that is required for cytoplasmic steps in 40S subunit maturation. S. cerevisiae Tsr1 was expressed as an N-terminal GST fusion protein in E. coli. A mutant Tsr1ΔNΔloop was generated in which residues 410 to 476 of Tsr1 were replaced with a short glycine and serine rich sequence; in addition, the N-ternimal 45 amino acid residues were replaced by five amino acid residues “GPDSD”. Tsr1ΔNΔloop allowed for generating native crystals that diffracted to 3.6 Å. Here we characterize both wild type Tsr1 and Tsr1ΔNΔloop using cross-linking/mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:29:02.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Chen
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-28 07:49:13	ID requested
1	2017-02-08 04:51:08	announced
⏵ 2	2024-10-22 04:29:04	announced	2024-10-22: Updated project metadata.

10.1038/ncomms11789;

McCaughan UM, Jayachandran U, Shchepachev V, Chen ZA, Rappsilber J, Tollervey D, Cook AG, Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases. Nat Commun, 7():11789(2016) [pubmed]

curator keyword: Biological

submitter keyword: Pre-40S ribosome biogenesis, protein structure, cross-linking/mass spectrometry

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, UK Department of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Germany
contact email	juri.rappsilber@ed.ac.uk
lab head
Zhuo Chen
contact affiliation	Technischen Universität Berlin
contact email	zhuo.chen@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004074

PRIDE project URI

• PRIDE
  1. PXD004074
     1. Label: PRIDE project
     2. Name: Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases