PXD004073

PXD004073 is an original dataset announced via ProteomeXchange.

Title	iTRAQ quantification of hippocampal proteins in a mouse model of unpredictable maternal separation and maternal stress
Description	Adverse experiences in early life are risk factors for the development of behavioral and physiological symptoms that can lead to psychiatric and cognitive disorders later in life. Some of these symptoms can be transmitted to the offspring, in some cases by non-genomic mechanisms involving germ cells. Using a mouse model of unpredictable maternal separation and maternal stress, we show that postnatal trauma alters coping behaviors in adverse conditions in exposed males when adult and in their adult male progeny. The behavioral changes are accompanied by increased glucocorticoid receptor (GR) expression and decreased DNA methylation of the GR promoter in the hippocampus. DNA methylation is also decreased in sperm cells of exposed males when adult. Transgenerational transmission of behavioral symptoms is prevented by paternal environmental enrichment, an effect associated with the reversal of alterations in GR gene expression and DNA methylation in the hippocampus of the male offspring. These findings highlight the influence of both, negative and positive environmental factors on behavior across generations, and the plasticity of the epigenome across life.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_06:21:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Mizzi Brunner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-28 06:18:27	ID requested
⏵ 1	2018-10-26 06:21:12	announced

Gapp K, Bohacek J, Grossmann J, Brunner AM, Manuella F, Nanni P, Mansuy IM, Potential of Environmental Enrichment to Prevent Transgenerational Effects of Paternal Trauma. Neuropsychopharmacology, 41(11):2749-58(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: paternal trauma, trangenerational inheritance, environmental enrichment, iTRAQ, mouse, hippocampus

Isabelle M Mansuy
contact affiliation	ETH Zürich, D-HEST
contact email	imansuy@ethz.ch
lab head
Andrea Mizzi Brunner
contact affiliation	Utrecht University
contact email	a.m.brunner@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004073
     1. Label: PRIDE project
     2. Name: iTRAQ quantification of hippocampal proteins in a mouse model of unpredictable maternal separation and maternal stress