PXD004069

PXD004069 is an original dataset announced via ProteomeXchange.

Title	Sus scrofa (pig) small intestinal mucosa LC-MS/MS
Description	In this study, we applied the isobaric tags for relative and absolute quantitation (iTRAQ) technique to detect alterations in the proteomic profile of the jejunal mucosa using a porcine model in which piglets were offered the protein-limited (PL) diet. Protein identification and quantification for iTRAQ experiments were performed using ProteinPilot (v4.0.8085) software. The LC-MS/MS data were searched against the UniProtKB (sus scrofa). To minimize the false discovery rate (FDR), a threshold for protein identification was applied, with the confident value > 95% (amount to the confident value “unused ProtScore” > 1.3 in ProteinPilot software), and at least one unique peptide was considered for protein identification. Proteins that were quantified with fold change > 2.0 were considered to be differentially expressed proteins. We identified 5275 proteins, 202 of which were differentially expressed. Furthermore, we adopted function annotation analysis of all identified proteins and function enrichment analysis of all differentially expressed proteins to explore more meaningful proteins and pathways.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:16:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingdong Yin
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-28 01:30:58	ID requested
1	2016-12-22 05:48:19	announced
⏵ 2	2017-10-24 03:16:59	announced	Updated project metadata.

Qin C, Qiu K, Sun W, Jiao N, Zhang X, Che L, Zhao H, Shen H, Yin J, A proteomic adaptation of small intestinal mucosa in response to dietary protein limitation. Sci Rep, 6():36888(2016) [pubmed]

curator keyword: Biological

submitter keyword: pig, jejunal mucosa, LC-MS/MS

Jingdong Yin, State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University
contact affiliation	Jingdong Yin, State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University
contact email	yinjd@cau.edu.cn
lab head
Jingdong Yin
contact affiliation	State Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University
contact email	yinjd@cau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004069

PRIDE project URI

• PRIDE
  1. PXD004069
     1. Label: PRIDE project
     2. Name: Sus scrofa (pig) small intestinal mucosa LC-MS/MS