PXD004062

PXD004062 is an original dataset announced via ProteomeXchange.

Title	Changes in the membrane proteome of exosomes released from human macrophages after infection with Mycobacterium tuberculosis.
Description	One of the greatest obstacles to eliminating TB is the lack of predictive and diagnostic biomarkers. Exosomes are a promising alternative source of biomarkers for TB since they can carry mycobacterial antigens. We hypothesize that exosomes from Mtb-infected macrophages exhibit a characteristic selection of human protein that can be evaluated as TB biomarkers. We looked for the localization—exosomal membrane or lumen—of the differentially abundant proteins. Exosomes were obtained from THP-1-derived macrophages (Mtb infected and uninfected controls). The exosome population was validated by nanoparticle tracking analysis and western blot. The protein composition of exosomes was evaluated by tandem mass spectrometry. Differences in protein composition and abundances between exosomes from infected and control cells were evaluated by t-test the proteomics findings were confirmed by western blot. Using a biotinylation strategy we verified the protein localization. Forty-seven proteins were significantly more abundant in exosomes from Mtb-infected cells, 66% were predicted to be membrane associated. The biotinylation pattern confirmed the membrane-association of some of these proteins.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_03:10:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004062
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gustavo Diaz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-27 02:05:08	ID requested
1	2016-12-20 14:13:33	announced
⏵ 2	2017-10-24 03:10:15	announced	Updated project metadata.

Diaz G, Wolfe LM, Kruh-Garcia NA, Dobos KM, Changes in the Membrane-Associated Proteins of Exosomes Released from Human Macrophages after Mycobacterium tuberculosis Infection. Sci Rep, 6():37975(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium tuberculosis

exosomes

membrane proteins

macrophage

Karen Dobos
contact affiliation	Director, Research Integrity and Compliance Review Office (http://ricro.colostate.edu/) Associate Professor, Mycobacteria Research Laboratories Dept of Microbiology, Immunology,& Pathology (http://www.cvmbs.colostate.edu/DirectorySearch/Search/MemberProfile/CVMBS/3607) Colorado State University
contact email	karen.dobos@colostate.edu
lab head
Gustavo Diaz
contact affiliation	Colorado State University
contact email	diaz.gustavo2011@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004062

PRIDE project URI

• PRIDE
  1. PXD004062
     1. Label: PRIDE project
     2. Name: Changes in the membrane proteome of exosomes released from human macrophages after infection with Mycobacterium tuberculosis.