PXD004061 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bathymodiolus azoricus host-symbiont proteomics |
Description | Bathymodiolus azoricus is a deep-sea mussel found in the hydrothermal vent fields of the Mid-Atlantic Ridge. It lives in symbiosis with sulfur- and methane-oxidizing γ-proteobacteria within its gills. In our study, we aimed to understand the metabolic and physiological interconnections between the symbiotic partners. For this purpose, symbionts and host were physically separated using density gradient centrifugation. This procedure yielded a symbiont-enriched gradient pellet fraction and a supernatant fraction enriched in host components. The cytosolic and membrane-associated proteome of both these fractions along with whole gill and foot tissue of the mussel were then investigated through 1D-PAGE LC-MS/MS. Proteins were quantified based on their spectral counts using the NSAF method. For efficient identification, sequences from evolutionarily related endosymbiotic and free-living bacteria and from bivalve host relatives were compiled into a comprehensive protein database. A total of 3178 host and symbiont proteins were identified from all samples. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_05:52:23.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004061 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ruby Ponnudurai |
SpeciesList | scientific name: methanotrophic endosymbiont of Bathymodiolus azoricus (Menez Gwen); NCBI TaxID: 343232; scientific name: thiotrophic endosymbiont of Bathymodiolus azoricus (Menez Gwen); NCBI TaxID: 343237; scientific name: Bathymodiolus azoricus (Mussel); NCBI TaxID: 150808; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-04-27 01:58:46 | ID requested | |
1 | 2016-10-06 08:17:47 | announced | |
2 | 2016-10-07 05:45:16 | announced | Updated project metadata. |
3 | 2016-11-17 05:42:06 | announced | Updated project metadata. |
4 | 2017-10-24 03:07:59 | announced | Updated project metadata. |
⏵ 5 | 2017-10-24 05:52:25 | announced | Updated project metadata. |
Publication List
Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM, Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife, 4():e07966(2015) [pubmed] |
Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S, Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J, 11(2):463-477(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Bathymodiolus, symbiont, host, proteomics, LC-MS/MS |
Contact List
Thomas Schweder, Stephanie Markert |
contact affiliation | University of Greifswald, Institut für Pharmazie/C_DAT, Pharmazeutische Biotechnologie, Felix-Hausdorff-Str. 3, 17489 Greifswald |
contact email | stephanie.markert@uni-greifswald.de |
lab head | |
Ruby Ponnudurai |
contact affiliation | University of Greifswald |
contact email | rp114410@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004061
- Label: PRIDE project
- Name: Bathymodiolus azoricus host-symbiont proteomics