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PXD004061

PXD004061 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBathymodiolus azoricus host-symbiont proteomics
DescriptionBathymodiolus azoricus is a deep-sea mussel found in the hydrothermal vent fields of the Mid-Atlantic Ridge. It lives in symbiosis with sulfur- and methane-oxidizing γ-proteobacteria within its gills. In our study, we aimed to understand the metabolic and physiological interconnections between the symbiotic partners. For this purpose, symbionts and host were physically separated using density gradient centrifugation. This procedure yielded a symbiont-enriched gradient pellet fraction and a supernatant fraction enriched in host components. The cytosolic and membrane-associated proteome of both these fractions along with whole gill and foot tissue of the mussel were then investigated through 1D-PAGE LC-MS/MS. Proteins were quantified based on their spectral counts using the NSAF method. For efficient identification, sequences from evolutionarily related endosymbiotic and free-living bacteria and from bivalve host relatives were compiled into a comprehensive protein database. A total of 3178 host and symbiont proteins were identified from all samples.
HostingRepositoryPRIDE
AnnounceDate2017-10-24
AnnouncementXMLSubmission_2017-10-24_05:52:23.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004061
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRuby Ponnudurai
SpeciesList scientific name: methanotrophic endosymbiont of Bathymodiolus azoricus (Menez Gwen); NCBI TaxID: 343232; scientific name: thiotrophic endosymbiont of Bathymodiolus azoricus (Menez Gwen); NCBI TaxID: 343237; scientific name: Bathymodiolus azoricus (Mussel); NCBI TaxID: 150808;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-04-27 01:58:46ID requested
12016-10-06 08:17:47announced
22016-10-07 05:45:16announcedUpdated project metadata.
32016-11-17 05:42:06announcedUpdated project metadata.
42017-10-24 03:07:59announcedUpdated project metadata.
52017-10-24 05:52:25announcedUpdated project metadata.
Publication List
Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM, Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife, 4():e07966(2015) [pubmed]
Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S, Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J, 11(2):463-477(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Bathymodiolus, symbiont, host, proteomics, LC-MS/MS
Contact List
Thomas Schweder, Stephanie Markert
contact affiliationUniversity of Greifswald, Institut für Pharmazie/C_DAT, Pharmazeutische Biotechnologie, Felix-Hausdorff-Str. 3, 17489 Greifswald
contact emailstephanie.markert@uni-greifswald.de
lab head
Ruby Ponnudurai
contact affiliationUniversity of Greifswald
contact emailrp114410@uni-greifswald.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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