PXD004044

PXD004044 is an original dataset announced via ProteomeXchange.

Title	Comparison of hair bundle proteomes from WT and Pls1-knockout mouse utricles
Description	With their stereotyped dimensions and orderly arrangement, as well as their essential role in auditory and vestibular function, stereocilia of sensory hair cells provide a particularly prominent example of the importance of cellular actin protrusions. Uniformity of actin packing in stereocilia is mediated by actin crosslinkers of the plastin, fascin, and espin families. While mice lacking ESPN (espin) have no vestibular function, we found that mice that either lacked PLS1 (plastin 1) or had nonfunctional FSCN2 (fascin 2) had vestibular function that was modestly reduced, with double-mutant mice being more strongly affected. Despite its outsized importance in functional assays, targeted mass spectrometry indicated that ESPN only accounted for ~15% of the total crosslinkers in mouse utricle stereocilia. PLS1 was the most abundant of the crosslinkers, and the second-most-abundant protein overall in stereocilia. Stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, while wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. Stereocilia with normal PLS1 but that lacked functional FSCN2 had normal liquid packing and normal dimensions. All three crosslinkers are required for normal stereocilia structure and function, but PLS1 biases stereocilia towards liquid packing, which allows stereocilia to grow to greater diameter.
HostingRepository	PRIDE
AnnounceDate	2016-09-28
AnnouncementXML	Submission_2016-09-28_02:36:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barr-Gillespie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-25 02:33:38	ID requested
⏵ 1	2016-09-28 02:37:00	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Hair cells, stereocilia, utricle, vestibular, plastin, fimbrin, fascin, espin

Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland OR 97239 USA
contact email	gillespp@ohsu.edu
lab head
Peter Barr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004044

PRIDE project URI

• PRIDE
  1. PXD004044
     1. Label: PRIDE project
     2. Name: Comparison of hair bundle proteomes from WT and Pls1-knockout mouse utricles