PXD004043

PXD004043 is an original dataset announced via ProteomeXchange.

Title	Time-course Activation of Human Peripheral Blood Eosinophils by Cytokines
Description	Activated eosinophils contribute to airway dysfunction and tissue remodeling in asthma, and thus are considered an important factor in asthma pathology. We report here comparative proteomic and phosphoproteomic changes upon activation of eosinophils using eight cytokines individually and in selected cytokine combinations, in time-course reactions. Differential protein and phosphoprotein expressions were determined by mass spectrometry after 2-dimensional gel electrophoresis (2DGE) followed by protein identification by MS, and by label-free LC-MS/MS. We found that each cytokine-stimulation produced significantly different changes in the eosinophil proteome and phosphoproteome, with phosphoproteomic changes being more pronounced and having an earlier onset. Furthermore, we observed that IL-5, GM-CSF, and IL 3 showed the greatest change in protein expression and phosphorylation, and this expression differed markedly from those of the other five cytokines evaluated. Comprehensive univariate and multivariate statistical analyses were employed to evaluate the comparative results. We also monitored eosinophil activation using flow cytometry (FC) analysis of CD69 expression. In agreement with our proteomic studies, FC indicated that IL-5, GM-CSF, and IL-3 were more effective than the other five cytokines studied in stimulating a cell surface CD69 increase indicative of eosinophil activation. Moreover, selected combinations of cytokines revealed proteomic patterns with many proteins in common with single cytokine expression patterns but also showed a greater than additive effect of the two cytokines employed, indicating a more complex signaling pathway that was reflective of a more typical inflammatory pathology.
HostingRepository	PRIDE
AnnounceDate	2017-12-19
AnnouncementXML	Submission_2017-12-18_23:49:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kizhake Soman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	AB SCIEX instrument model; Orbitrap Fusion; TOF/TOF 5800

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-25 02:17:57	ID requested
⏵ 1	2017-12-18 23:49:50	announced

Soman KV, Stafford SJ, Pazdrak K, Wu Z, Luo X, White WI, Wiktorowicz JE, Calhoun WJ, Kurosky A, Activation of Human Peripheral Blood Eosinophils by Cytokines in a Comparative Time-Course Proteomic/Phosphoproteomic Study. J Proteome Res, 16(8):2663-2679(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Eosinophils, cytokines, proteomics, phosphoproteomics, activation, mass spectrometry, flow cytometry, asthma

Alexander Kurosky, Ph.D.
contact affiliation	Professor, Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX
contact email	akurosky@utmb.edu
lab head
Kizhake Soman
contact affiliation	Department of Biochemistry & Molecular Biology
contact email	kvsoman@utmb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD004043

PRIDE project URI

• PRIDE
  1. PXD004043
     1. Label: PRIDE project
     2. Name: Time-course Activation of Human Peripheral Blood Eosinophils by Cytokines