PXD004034

PXD004034 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells
Description	CD14+ Monocytes from healthy volunteers were purified by MACS (negative selection) and FACSorting and either left untreated or stimulated for 24h and 48h with LPS. THP-1 cells were stimulated for 4h, 24h and 48h with LPS. Glycoproteins were captured with hydrazide chemistry and tryptic and PNGase F-released peptide fractions analyzed by MS/MS. Quantitative assessment revealed differential glycoprotein expression in activated/LPS-tolerized monocytes and naïve monocytes and THP-1 cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:49.393.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Müller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-22 04:37:03	ID requested
1	2017-04-24 06:04:05	announced
⏵ 2	2024-10-22 04:28:56	announced	2024-10-22: Updated project metadata.

M, ü, ller MM, Lehmann R, Klassert TE, Reifenstein S, Conrad T, Moore C, Kuhn A, Behnert A, Guthke R, Driesch D, Slevogt H, expression. Sci Rep, 7(1):838(2017) [pubmed]

10.1038/s41598-017-00828-y;

submitter keyword: monocytes glycoproteomics

Hortense Slevogt
contact affiliation	ZIK Septomics, University Hospital Jena, Germany
contact email	hortense.slevogt@med.uni-jena.de
lab head
Mario Müller
contact affiliation	Host Septomics - ZIK Septomics Jena University Hosptial Jena Germany
contact email	mario.mueller1@med.uni-jena.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD004034

PRIDE project URI

• PRIDE
  1. PXD004034
     1. Label: PRIDE project
     2. Name: Glycoproteomes of naive and LPS stimulated human monocytes and THP-1 cells