PXD004027

PXD004027 is an original dataset announced via ProteomeXchange.

Title	Variation in auxin sensing guides AUX/IAA repressor ubiquitylation and destruction
Description	Auxin is a drug-like small molecule and morphogen that triggers the formation of SCFTIR1/AFB-AUX/IAA co-receptor complexes leading to ubiquitylation and proteasome-dependent degradation of AUX/IAA transcriptional repressors in plants. Here, we systematically dissect auxin sensing by SCFTIR1-IAA6 and SCFTIR1-IAA19 co-receptor complexes, and assess IAA6/IAA19 ubiquitylation in vitro and IAA6/IAA19 degradation in vivo. TIR1-IAA19 and TIR1-IAA6 interactions form co-receptors with different affinities, which specify ubiquitylation and turnover rate of the AUX/IAA. We demonstrate lysine ubiquitylation in IAA6/IAA19 and propose this ubiquitylation signature to be a consequence of auxin-mediated SCFTIR1-AUX/IAA interactions. We present evidence for an evolving AUX/IAA repertoire, typified by the IAA6/IAA19 ohnologs, that contributes differentially to auxin sensing. We postulate that AUX/IAAs have emerged as a versatility toolbox for auxin-modulated transcriptional control, as the gamut of AUX/IAA destruction kinetics enables fine-tuning of transcriptional auxin response and contributes to the complexity of hormone signaling.
HostingRepository	PRIDE
AnnounceDate	2017-06-14
AnnouncementXML	Submission_2017-06-14_00:26:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004027
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wolfgang Hoehenwarter
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-21 04:18:36	ID requested
⏵ 1	2017-06-14 00:26:47	announced

Winkler M, Niemeyer M, Hellmuth A, Janitza P, Christ G, Samodelov SL, Wilde V, Majovsky P, Trujillo M, Zurbriggen MD, Hoehenwarter W, Quint M, Calder, ó, n Villalobos LIA, Variation in auxin sensing guides AUX/IAA transcriptional repressor ubiquitylation and destruction. Nat Commun, 8():15706(2017) [pubmed]

curator keyword: Biological

submitter keyword: auxin, phytohormone, proteolysis, ubiquitination, ubiquitylation, SCF-type E3 ligase, AUX/IAA, TIR1/AFBs

Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
lab head
Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry
contact email	wolfgang.hoehenwarter@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004027
     1. Label: PRIDE project
     2. Name: Variation in auxin sensing guides AUX/IAA repressor ubiquitylation and destruction