PXD004026

PXD004026 is an original dataset announced via ProteomeXchange.

Title	Clickable Glutathione Approach for Identification of Protein Glutathionylation in Response to Glucose Metabolism
Description	Glucose metabolism and mitochondrial function are closely interconnected with cellular redox-homeostasis. Although glucose starvation, which often occurs in ischemic heart or insufficient vascularization of tumor cells, is known to induce redox-disturbance, global and individual protein glutathionylation in response to glucose metabolism or mitochondrial activity remains largely unknown. In this report, we use our clickable glutathione approach, which form clickable glutathione (azido-glutathione) by using a mutant of glutathione synthetase (GS M4), for detection and identification of protein glutathionylation in response to glucose starvation. We found that protein glutathionylation is readily induced in response to glucose starvation when mitochondrial reactive oxygen species (ROS) are elevated in cells, and glucose is the major determinant for inducing reversible glutathionylation. Proteomic and biochemical analysis identified over 1,300 proteins, including SMYD2, PP2Cα, and catalase. We further analyzed the cysteine modification site and functional implication of PP2Cα glutathionylation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:29.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eranthie Weerapana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-04-21 02:51:45	ID requested
1	2018-10-26 06:14:57	announced
⏵ 2	2024-10-22 04:28:35	announced	2024-10-22: Updated project metadata.

10.1039/c6mb00175k;

Samarasinghe KT, Munkanatta Godage DN, Zhou Y, Ndombera FT, Weerapana E, Ahn YH, A clickable glutathione approach for identification of protein glutathionylation in response to glucose metabolism. Mol Biosyst, 12(8):2471-80(2016) [pubmed]

curator keyword: Biological

submitter keyword: Glutathionylation

Eranthie Weerapana
contact affiliation	Boston College
contact email	eranthie@bc.edu
lab head
Eranthie Weerapana
contact affiliation	Boston College
contact email	eranthie@bc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004026

PRIDE project URI

• PRIDE
  1. PXD004026
     1. Label: PRIDE project
     2. Name: Clickable Glutathione Approach for Identification of Protein Glutathionylation in Response to Glucose Metabolism